About: Chemiluminescent determination of reactive oxygen species using Pholasin luminophore in birds and insect phagocytes

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About: Chemiluminescent determination of reactive oxygen species using Pholasin luminophore in birds and insect phagocytes Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Phagocytosis is one of the most important innate immunity mechanisms which prevents organism against pathogens overcoming the natural barriers. There are several methods widely used for evaluation of phagocytosis efficiency. One of them is to measure the level of reactive oxygen species (ROS) produced by phagocytes - respiratory burst of phagocytes. ROS production can be elicited by addition of activators such as lipopolysaccharide (LPS), zymosan, starch particles, phorbol-12-miristate-13-acetate (PMA) or N-formyl-methionyl-leucyl-phenylalanin (fMLP). Produced ROS cause oxidation of luminophore which subsequently emits the energy in form of light measured by luminometers. Luminol is the most widely used luminophore. On the other hand evaluation of respiratory burst using this method on birds or insect samples is not possible due to a low production of ROS and low sensitivity of luminol. Phagocytosis is one of the most important innate immunity mechanisms which prevents organism against pathogens overcoming the natural barriers. There are several methods widely used for evaluation of phagocytosis efficiency. One of them is to measure the level of reactive oxygen species (ROS) produced by phagocytes - respiratory burst of phagocytes. ROS production can be elicited by addition of activators such as lipopolysaccharide (LPS), zymosan, starch particles, phorbol-12-miristate-13-acetate (PMA) or N-formyl-methionyl-leucyl-phenylalanin (fMLP). Produced ROS cause oxidation of luminophore which subsequently emits the energy in form of light measured by luminometers. Luminol is the most widely used luminophore. On the other hand evaluation of respiratory burst using this method on birds or insect samples is not possible due to a low production of ROS and low sensitivity of luminol. (en)
Title	Chemiluminescent determination of reactive oxygen species using Pholasin luminophore in birds and insect phagocytes Chemiluminescent determination of reactive oxygen species using Pholasin luminophore in birds and insect phagocytes (en)
skos:prefLabel	Chemiluminescent determination of reactive oxygen species using Pholasin luminophore in birds and insect phagocytes Chemiluminescent determination of reactive oxygen species using Pholasin luminophore in birds and insect phagocytes (en)
skos:notation	RIV/00216224:14310/14:00073254!RIV15-MSM-14310___
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(QJ1210047)
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Prokopová, Lucie Vojtek, Libor Hyršl, Pavel Dobeš, Pavel
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	7108
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/14:00073254
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Chemiluminiscence; phagocytes; Pholasin; reactive oxygen species (en)
http://linked.open.../riv/klicoveSlovo	Chemiluminiscence reactive oxygen species Pholasin phagocytes
http://linked.open...ontrolniKodProRIV	[171C7478E72E]
http://linked.open...in/vavai/riv/obor	EC
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Research and development of novel agents for honey bee health improvement
http://linked.open...UplatneniVysledku	2014
http://linked.open...iv/tvurceVysledku	Dobeš, Pavel Hyršl, Pavel Vinkler, Michal Vojtek, Libor Prokopová, Lucie Vinklerova, Jitka
http://localhost/t...ganizacniJednotka	14310

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