About: HPLC profiles of polyphenolic compounds and estimation of antioxidant potential in sea buckthorn plant parts

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Predominant medical effect of many plants and herbs is related to the presence of polyphenolic secondary plant metabolites commonly known as flavonoids. Different classes of flavonoids are synthesized as a response to the ambient stimuli (climate, stress, mold attacks etc.) and accumulate in various cells and plant parts. The antioxidant potential and selected flavonoids content in sea buckthorn (Hippophaë Rhamnoides L.) plant part materials (stems, leaves and fruits) has been investigated during one vegetative cycle. New RP-HPLC method using gradient elution for determination of 10 polyphenols, namely catechin, epicatechin, rutin, quercetin, quercitrin, kaempferol, gallic acid, p-coumaric acid, caffeic acid and ferulic acid has been developed. Significant part of method development included optimization of extraction step for sample matrix removal. As the most suitable, liquid-liquid extraction of methanolic plant solutions using diethylether was found. Predominant medical effect of many plants and herbs is related to the presence of polyphenolic secondary plant metabolites commonly known as flavonoids. Different classes of flavonoids are synthesized as a response to the ambient stimuli (climate, stress, mold attacks etc.) and accumulate in various cells and plant parts. The antioxidant potential and selected flavonoids content in sea buckthorn (Hippophaë Rhamnoides L.) plant part materials (stems, leaves and fruits) has been investigated during one vegetative cycle. New RP-HPLC method using gradient elution for determination of 10 polyphenols, namely catechin, epicatechin, rutin, quercetin, quercitrin, kaempferol, gallic acid, p-coumaric acid, caffeic acid and ferulic acid has been developed. Significant part of method development included optimization of extraction step for sample matrix removal. As the most suitable, liquid-liquid extraction of methanolic plant solutions using diethylether was found. (en)
Title	HPLC profiles of polyphenolic compounds and estimation of antioxidant potential in sea buckthorn plant parts HPLC profiles of polyphenolic compounds and estimation of antioxidant potential in sea buckthorn plant parts (en)
skos:prefLabel	HPLC profiles of polyphenolic compounds and estimation of antioxidant potential in sea buckthorn plant parts HPLC profiles of polyphenolic compounds and estimation of antioxidant potential in sea buckthorn plant parts (en)
skos:notation	RIV/00216224:14310/13:00070150!RIV14-MSM-14310___
http://linked.open...avai/riv/aktivita	S
http://linked.open...avai/riv/aktivity	S
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Kubáň, Vlastimil Roblová, Vendula Bittová, Miroslava Krejzová, Eliška
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	78191
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/13:00070150
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	HPLC; polyphenolics; antioxidant potential; sea buckthorn (en)
http://linked.open.../riv/klicoveSlovo	antioxidant potential polyphenolics HPLC sea buckthorn
http://linked.open...ontrolniKodProRIV	[1195F00EB095]
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...UplatneniVysledku	2013
http://linked.open...iv/tvurceVysledku	Bittová, Miroslava Kubáň, Vlastimil Roblová, Vendula Krejzová, Eliška
http://localhost/t...ganizacniJednotka	14310

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