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  • A new capillary zone electrophoretic method was developed for the determination of twelve purine and pyrimidine nucleotides, two adenine coenzymes and their reduced forms, and acetyl coenzyme A in various cell extracts. As the concentration levels of these metabolites in living cells are low; the capillary zone electrophoresis was combined with field-enhanced sample stacking. As a result, the separation conditions were optimised to achieve a suitable resolution at the relatively high sample volume provided by this on-line pre-concentration technique.
  • A new capillary zone electrophoretic method was developed for the determination of twelve purine and pyrimidine nucleotides, two adenine coenzymes and their reduced forms, and acetyl coenzyme A in various cell extracts. As the concentration levels of these metabolites in living cells are low; the capillary zone electrophoresis was combined with field-enhanced sample stacking. As a result, the separation conditions were optimised to achieve a suitable resolution at the relatively high sample volume provided by this on-line pre-concentration technique. (en)
Title
  • Simultaneous quantification of energetically important metabolites in various cell types by capillary zone electrophoresis
  • Simultaneous quantification of energetically important metabolites in various cell types by capillary zone electrophoresis (en)
skos:prefLabel
  • Simultaneous quantification of energetically important metabolites in various cell types by capillary zone electrophoresis
  • Simultaneous quantification of energetically important metabolites in various cell types by capillary zone electrophoresis (en)
skos:notation
  • RIV/00216224:14310/13:00066386!RIV14-MSM-14310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(EE2.3.20.0182), P(GAP206/11/0009)
http://linked.open...iv/cisloPeriodika
  • 23
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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  • 105312
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/13:00066386
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  • metabolomics; nucleotides; Paracoccus denitrificans; stem cells; system biology (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV
  • [363EC4B3A563]
http://linked.open...i/riv/nazevZdroje
  • Journal of Separation Science
http://linked.open...in/vavai/riv/obor
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http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 36
http://linked.open...iv/tvurceVysledku
  • Klejdus, Bořivoj
  • Glatz, Zdeněk
  • Musilová, Jindra
http://linked.open...ain/vavai/riv/wos
  • 000327556300021
issn
  • 1615-9306
number of pages
http://bibframe.org/vocab/doi
  • 10.1002/jssc.201300926
http://localhost/t...ganizacniJednotka
  • 14310
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