About: Are Time-Dependent Fluorescence Shifts at the Tunnel Mouth of Haloalkane Dehalogenase Enzymes Dependent on the Choice of the Chromophore?

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	AB Time-dependent fluorescence shifts (TDFS) of chromophores selectively attached to proteins may give information on the dynamics of the probed protein moieties and their degree of hydration. Previously, we demonstrated that a coumarin dye selectively labeling the tunnel mouth of different haloalkane dehalogenases (HLDs) can distinguish between different widths of tunnel mouth openings. In order to generalize those findings analogous experiments were performed using a different chromophore probing the same region of these enzymes. To this end we synthesized and characterized three new fluorescent probes derived from dimethylaminonaphthalene bearing a linker almost identical to that of the coumarin dye used in our previous study. AB Time-dependent fluorescence shifts (TDFS) of chromophores selectively attached to proteins may give information on the dynamics of the probed protein moieties and their degree of hydration. Previously, we demonstrated that a coumarin dye selectively labeling the tunnel mouth of different haloalkane dehalogenases (HLDs) can distinguish between different widths of tunnel mouth openings. In order to generalize those findings analogous experiments were performed using a different chromophore probing the same region of these enzymes. To this end we synthesized and characterized three new fluorescent probes derived from dimethylaminonaphthalene bearing a linker almost identical to that of the coumarin dye used in our previous study. (en)
Title	Are Time-Dependent Fluorescence Shifts at the Tunnel Mouth of Haloalkane Dehalogenase Enzymes Dependent on the Choice of the Chromophore? Are Time-Dependent Fluorescence Shifts at the Tunnel Mouth of Haloalkane Dehalogenase Enzymes Dependent on the Choice of the Chromophore? (en)
skos:prefLabel	Are Time-Dependent Fluorescence Shifts at the Tunnel Mouth of Haloalkane Dehalogenase Enzymes Dependent on the Choice of the Chromophore? Are Time-Dependent Fluorescence Shifts at the Tunnel Mouth of Haloalkane Dehalogenase Enzymes Dependent on the Choice of the Chromophore? (en)
skos:notation	RIV/00216224:14310/13:00065798!RIV14-GA0-14310___
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(ED1.100/02/0123), P(GAP207/12/0775), P(GBP208/12/G016), P(IAA401630901)
http://linked.open...iv/cisloPeriodika	26
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Paruch, Kamil Maier, Lukáš Kováčová, Silvia Damborský, Jiří Brezovský, Jan Chaloupková, Radka
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	62049
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/13:00065798
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	DYNAMIC STOKES SHIFT; WATER-PROTEIN FLUCTUATIONS; POLAR SOLVATION DYNAMICS; HYDRATION DYNAMICS; DIELECTRIC RESPONSE; SOLVENT RELAXATION; ACTIVE-SITE; SUBSTRATE; SURFACE; SIMULATIONS (en)
http://linked.open.../riv/klicoveSlovo	DIELECTRIC RESPONSE DYNAMIC STOKES SHIFT HYDRATION DYNAMICS POLAR SOLVATION DYNAMICS SOLVENT RELAXATION SUBSTRATE WATER-PROTEIN FLUCTUATIONS SURFACE ACTIVE-SITE SIMULATIONS
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[CA7346D4F35D]
http://linked.open...i/riv/nazevZdroje	Journal of Physical Chemistry B
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...vavai/riv/projekt	Controlling structure and function of biomolecules at the molecular scale: theory meets experiment Strukturně-funkční vztahy haloalkan dehalogenas Evolution of substrate specificity in enzymes acting on xenobiotic compounds St. Anne´s University Hospital Brno - International Clinical Research Center (FNUSA-ICRC)
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	117
http://linked.open...iv/tvurceVysledku	Brezovský, Jan Chaloupková, Radka Damborský, Jiří Hof, Martin Paruch, Kamil Maier, Lukáš Amaro, Mariana Kováčová, Silvia Sykora, Jan
http://linked.open...ain/vavai/riv/wos	000321542200006
issn	1520-6106
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1021/jp403708c
http://localhost/t...ganizacniJednotka	14310

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