About: Bioluminescence determination of antibacterial activity of Bombyx mori and Galleria mellonella haemolymph     Goto   Sponge   NotDistinct   Permalink

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  • We describe an antibacterial assay based on bioluminescence of two Gram negative bacteria Photorhabdus luminescens and transformed Escherichia coli, which can be used for a real-time measurement of antibacterial activity. We observed a significant dose-dependent decrease of bioluminescence using both bacterial species during one hour after exposure to Bombyx mori or Galleria mellonella haemolymph. The humoral origin of the antibacterial activity observed in whole haemolymph was confirmed in haemolymph plasma without haemocytes. Antibacterial activity operating against Gram negative bacteria was measured in unaffected insect larvae as well as after septic injury; increased antibacterial activity in haemolymph was detected in the latter case which confirms inducibility of antimicrobial agents. This method can be widely used for determination of antibacterial activity in insects and supposedly in other invertebrates.
  • We describe an antibacterial assay based on bioluminescence of two Gram negative bacteria Photorhabdus luminescens and transformed Escherichia coli, which can be used for a real-time measurement of antibacterial activity. We observed a significant dose-dependent decrease of bioluminescence using both bacterial species during one hour after exposure to Bombyx mori or Galleria mellonella haemolymph. The humoral origin of the antibacterial activity observed in whole haemolymph was confirmed in haemolymph plasma without haemocytes. Antibacterial activity operating against Gram negative bacteria was measured in unaffected insect larvae as well as after septic injury; increased antibacterial activity in haemolymph was detected in the latter case which confirms inducibility of antimicrobial agents. This method can be widely used for determination of antibacterial activity in insects and supposedly in other invertebrates. (en)
Title
  • Bioluminescence determination of antibacterial activity of Bombyx mori and Galleria mellonella haemolymph
  • Bioluminescence determination of antibacterial activity of Bombyx mori and Galleria mellonella haemolymph (en)
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  • Bioluminescence determination of antibacterial activity of Bombyx mori and Galleria mellonella haemolymph
  • Bioluminescence determination of antibacterial activity of Bombyx mori and Galleria mellonella haemolymph (en)
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  • RIV/00216224:14310/13:00065681!RIV14-MZE-14310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • I, P(QJ1210047), S
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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http://linked.open...iv/duvernostUdaju
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  • 63489
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/13:00065681
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  • antibacterial activity; Galleria mellonella; Bombyx mori; bioluminescent bacteria (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [F7A635196197]
http://linked.open...v/mistoKonaniAkce
  • Zagreb
http://linked.open...i/riv/mistoVydani
  • Darmstadt
http://linked.open...i/riv/nazevZdroje
  • Insect pathogens and entomoparasitic nematodes
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
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http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Dobeš, Pavel
  • Hyršl, Pavel
  • Vojtek, Libor
  • Büyükgüzel, Ender
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
number of pages
http://purl.org/ne...btex#hasPublisher
  • IOBC-WPRS 2013
https://schema.org/isbn
  • 9789290672685
http://localhost/t...ganizacniJednotka
  • 14310
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