About: Bacteriophages of Staphylococcus aureus efficiently package various bacterial genes and mobile genetic elements including SCCmec with different frequencies

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Bacteriophages of Staphylococcus aureus efficiently package various bacterial genes and mobile genetic elements including SCCmec with different frequencies Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://onlinelibrary.wiley.com/doi/10.1111/j.1758-2229.2012.00378.x/abstract
Description	Staphylococcus aureus is a serious human and veterinary pathogen in which new strains with increasing virulence and antimicrobial resistance occur due to acquiring new genes by horizontal transfer. It is generally accepted that temperate bacteriophages play a major role in gene transfer. In this study, we proved the presence of various bacterial genes of the S. aureus COL strain directly within the phage particles via qPCR and quantified their packaging frequency. Nonparametric statistical analysis showed that transducing bacteriophages 11, 80 and 80lpha of serogroup B, in contrast to serogroup A bacteriophage 81, efficiently package selected chromosomal genes localized in 4 various loci of the chromosome and 8 genes carried on variable elements like staphylococcal cassette chromosome SCCmec, staphylococcal pathogenicity island SaPI1, genomic islands Saalpha and Sabeta, and plasmids with various frequency. Bacterial gene copy number per ng of DNA isolated from phage particles ranged between 1. Staphylococcus aureus is a serious human and veterinary pathogen in which new strains with increasing virulence and antimicrobial resistance occur due to acquiring new genes by horizontal transfer. It is generally accepted that temperate bacteriophages play a major role in gene transfer. In this study, we proved the presence of various bacterial genes of the S. aureus COL strain directly within the phage particles via qPCR and quantified their packaging frequency. Nonparametric statistical analysis showed that transducing bacteriophages 11, 80 and 80lpha of serogroup B, in contrast to serogroup A bacteriophage 81, efficiently package selected chromosomal genes localized in 4 various loci of the chromosome and 8 genes carried on variable elements like staphylococcal cassette chromosome SCCmec, staphylococcal pathogenicity island SaPI1, genomic islands Saalpha and Sabeta, and plasmids with various frequency. Bacterial gene copy number per ng of DNA isolated from phage particles ranged between 1. (en)
Title	Bacteriophages of Staphylococcus aureus efficiently package various bacterial genes and mobile genetic elements including SCCmec with different frequencies Bacteriophages of Staphylococcus aureus efficiently package various bacterial genes and mobile genetic elements including SCCmec with different frequencies (en)
skos:prefLabel	Bacteriophages of Staphylococcus aureus efficiently package various bacterial genes and mobile genetic elements including SCCmec with different frequencies Bacteriophages of Staphylococcus aureus efficiently package various bacterial genes and mobile genetic elements including SCCmec with different frequencies (en)
skos:notation	RIV/00216224:14310/13:00065568!RIV14-MZ0-14310___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA310/09/0459), P(NT12395)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Mužík, Jan Doškař, Jiří Růžičková, Vladislava Pantůček, Roman Mašlaňová, Ivana Varga, Marian Malúšková, Denisa
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	62811
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/13:00065568
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	bacteriophage transduction; horizontal gene transfer; Gram-positive bacteria; methicillin resistance; quantitative real-time PCR (en)
http://linked.open.../riv/klicoveSlovo	bacteriophage transduction methicillin resistance quantitative real-time PCR horizontal gene transfer Gram-positive bacteria
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[AB3A19B7785B]
http://linked.open...i/riv/nazevZdroje	Environmental Microbiology Reports
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	7 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Role of bacteriophages in horizontal transfer of virulence and drug resistance genes in Staphylococcus aureus Molecular detection and analysis of invasive small colony variants of S. aureus and resistant S. aureus strains from patients with cystic fibrosis
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	5
http://linked.open...iv/tvurceVysledku	Doškař, Jiří Pantůček, Roman Růžičková, Vladislava Mužík, Jan Mašlaňová, Ivana Kuntová, Lucie Malúšková, Denisa Varga, Marian
http://linked.open...ain/vavai/riv/wos	000314474500006
issn	1758-2229
number of pages	8 (xsd:int)
http://bibframe.org/vocab/doi	10.1111/j.1758-2229.2012.00378.x
http://localhost/t...ganizacniJednotka	14310

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 48 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software