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Description
  • Retention characteristics of selected synthetic 5-terminal phosphate absent penta-nucleotides containing adenine, guanine, and thymine were studied in relation to their sequence by hydrophilic interaction chromatography and ion-interaction reversed-phase liquid chromatography. We have found a simple linear additive model to describe the retention order in both separation modes in regard to their sequence. Our results show that the penta-nucleotide sequence, and thus its spatial arrangement induced by the surrounding environment, is highly related to the retention properties, so it may be hypothetically used to read the sequence from the retention properties acquired under particular separation conditions.
  • Retention characteristics of selected synthetic 5-terminal phosphate absent penta-nucleotides containing adenine, guanine, and thymine were studied in relation to their sequence by hydrophilic interaction chromatography and ion-interaction reversed-phase liquid chromatography. We have found a simple linear additive model to describe the retention order in both separation modes in regard to their sequence. Our results show that the penta-nucleotide sequence, and thus its spatial arrangement induced by the surrounding environment, is highly related to the retention properties, so it may be hypothetically used to read the sequence from the retention properties acquired under particular separation conditions. (en)
Title
  • Toward reading the sequence of short oligonucleotides from their retention factors obtained by means of hydrophilic interaction chromatography and ion-interaction reversed-phase liquid chromatography
  • Toward reading the sequence of short oligonucleotides from their retention factors obtained by means of hydrophilic interaction chromatography and ion-interaction reversed-phase liquid chromatography (en)
skos:prefLabel
  • Toward reading the sequence of short oligonucleotides from their retention factors obtained by means of hydrophilic interaction chromatography and ion-interaction reversed-phase liquid chromatography
  • Toward reading the sequence of short oligonucleotides from their retention factors obtained by means of hydrophilic interaction chromatography and ion-interaction reversed-phase liquid chromatography (en)
skos:notation
  • RIV/00216224:14310/12:00062072!RIV13-MSM-14310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • I, S
http://linked.open...iv/cisloPeriodika
  • 22
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 174672
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/12:00062072
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Hydrophilic interaction chromatography; Ion-interaction reversed-phase liquid chromatography; Oligonucleotide sequencing; Penta-nucleotide; Retention behavior (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV
  • [24A2503B40A0]
http://linked.open...i/riv/nazevZdroje
  • Journal of Separation Science
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 35
http://linked.open...iv/tvurceVysledku
  • Bittová, Miroslava
  • Trnková, Libuše
  • Havliš, Jan
  • Vrbková, Blanka
  • Fuksová, Hana
http://linked.open...ain/vavai/riv/wos
  • 000311415200023
issn
  • 1615-9306
number of pages
http://bibframe.org/vocab/doi
  • 10.1002/jssc.201200482
http://localhost/t...ganizacniJednotka
  • 14310
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