About: Identification and determination of relatedness of lactobacilli using different DNA amplification methods

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About: Identification and determination of relatedness of lactobacilli using different DNA amplification methods Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Several DNA amplification-based methods were used for identification and evaluation of the relation between lactobacilli isolated from breastfed full-term infant faeces, dairy products and silage. Twenty-seven strains isolated friom infant faeces were identified as Lactobacillus rhamnosus, Lactobacillus gasseri, Lactobacillus paracasei, Lactobacillus fermentum, Lactobacillus salivarius, Lactobacillus plantarum, and Lactobacillus helveticus using ten species-specific PCRs, multiplex PCR, and sequencing of 16SrDNA. Four strains were not identified. A repetitive extragenic palindromic PCR (rep-PCR) and randomly amplified polymorphic DNA PCR (RAPD-PCR) were used for confirmation of species identification. Fingerprints were used for evaluation of the relatedness of lactobacilli. Differences between strains from infant faeces, dairy products, and silage were not detected. Several DNA amplification-based methods were used for identification and evaluation of the relation between lactobacilli isolated from breastfed full-term infant faeces, dairy products and silage. Twenty-seven strains isolated friom infant faeces were identified as Lactobacillus rhamnosus, Lactobacillus gasseri, Lactobacillus paracasei, Lactobacillus fermentum, Lactobacillus salivarius, Lactobacillus plantarum, and Lactobacillus helveticus using ten species-specific PCRs, multiplex PCR, and sequencing of 16SrDNA. Four strains were not identified. A repetitive extragenic palindromic PCR (rep-PCR) and randomly amplified polymorphic DNA PCR (RAPD-PCR) were used for confirmation of species identification. Fingerprints were used for evaluation of the relatedness of lactobacilli. Differences between strains from infant faeces, dairy products, and silage were not detected. (en)
Title	Identification and determination of relatedness of lactobacilli using different DNA amplification methods Identification and determination of relatedness of lactobacilli using different DNA amplification methods (en)
skos:prefLabel	Identification and determination of relatedness of lactobacilli using different DNA amplification methods Identification and determination of relatedness of lactobacilli using different DNA amplification methods (en)
skos:notation	RIV/00216224:14310/12:00060656!RIV13-MSM-14310___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(2B06053), S, Z(MSM0021622415)
http://linked.open...iv/cisloPeriodika	9
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Španová, Alena Rittich, Bohuslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	140261
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/12:00060656
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Lactobacillus; breast fed children; dairy products; identification; PCR; fingerprinting (en)
http://linked.open.../riv/klicoveSlovo	Lactobacillus PCR identification breast fed children fingerprinting dairy products
http://linked.open...odStatuVydavatele	PL - Polská republika
http://linked.open...ontrolniKodProRIV	[149E8C30D51A]
http://linked.open...i/riv/nazevZdroje	Chemical Papers
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	New methods for the characterization and identification of probiotic bacterial strains suitable for functional foods.
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	66
http://linked.open...iv/tvurceVysledku	Rittich, Bohuslav Španová, Alena Turková, Kristýna
http://linked.open...ain/vavai/riv/wos	000305563300007
http://linked.open...n/vavai/riv/zamer	Molecular basis of cell and tissue regulations
issn	0366-6352
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.2478/s11696-012-0206-7
http://localhost/t...ganizacniJednotka	14310

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