About: INFLUENCE OF HEAT DENATURATION OF Beta-SHEET BREAKER PRION PROTEIN ON ELECTROCHEMICAL RESPONCE

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Electrochemical study of Beta-sheet breaker prion protein is the main aim of this study. For this purpose, cyclic voltammetry (CV), differential pulse voltammetry, differential pulse voltammetry Brdicka reaction and chronopotentiometric stripping analysis were used. Under the optimal conditions (phosphate buffer, pH 7.38 and time of accumulation 100 s), detected by DPV prion was characterized using different techniques and their limits of detection were found. Adsorptive transfer stripping technique coupled with the abovementioned methods offers very lower detection limits. The lowest limits of detection were determined by CPSA AdTS i.e. 25 pmol in volume of 5 microl. CPSA is therefore a very sensitive tool for the studying of prion behaviour. Moreover, the influence of heat denaturation was observed. It clearly follows from the results obtained that signals of prion decreased linearly depending on the duration of the heat treatment at 99 C for various time intervals: 0, 15, 30, 45, and 60 min. Electrochemical study of Beta-sheet breaker prion protein is the main aim of this study. For this purpose, cyclic voltammetry (CV), differential pulse voltammetry, differential pulse voltammetry Brdicka reaction and chronopotentiometric stripping analysis were used. Under the optimal conditions (phosphate buffer, pH 7.38 and time of accumulation 100 s), detected by DPV prion was characterized using different techniques and their limits of detection were found. Adsorptive transfer stripping technique coupled with the abovementioned methods offers very lower detection limits. The lowest limits of detection were determined by CPSA AdTS i.e. 25 pmol in volume of 5 microl. CPSA is therefore a very sensitive tool for the studying of prion behaviour. Moreover, the influence of heat denaturation was observed. It clearly follows from the results obtained that signals of prion decreased linearly depending on the duration of the heat treatment at 99 C for various time intervals: 0, 15, 30, 45, and 60 min. (en)
Title	INFLUENCE OF HEAT DENATURATION OF Beta-SHEET BREAKER PRION PROTEIN ON ELECTROCHEMICAL RESPONCE INFLUENCE OF HEAT DENATURATION OF Beta-SHEET BREAKER PRION PROTEIN ON ELECTROCHEMICAL RESPONCE (en)
skos:prefLabel	INFLUENCE OF HEAT DENATURATION OF Beta-SHEET BREAKER PRION PROTEIN ON ELECTROCHEMICAL RESPONCE INFLUENCE OF HEAT DENATURATION OF Beta-SHEET BREAKER PRION PROTEIN ON ELECTROCHEMICAL RESPONCE (en)
skos:notation	RIV/00216224:14310/12:00060129!RIV13-MSM-14310___
http://linked.open...avai/riv/aktivita	I
http://linked.open...avai/riv/aktivity	I
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Trnková, Libuše
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	141434
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/12:00060129
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	prion protein; cyclic voltammetry; differential pulse voltammetry; Brdicka reaction; chronopotentiometry (en)
http://linked.open.../riv/klicoveSlovo	cyclic voltammetry chronopotentiometry differential pulse voltammetry prion protein Brdicka reaction
http://linked.open...ontrolniKodProRIV	[F575080D096F]
http://linked.open...v/mistoKonaniAkce	Brno
http://linked.open...i/riv/mistoVydani	Brno
http://linked.open...i/riv/nazevZdroje	XII. Pracovní setkání fyzikálních chemiků a elektrochemiků. Sborník příspěvků.
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...UplatneniVysledku	2012
http://linked.open...iv/tvurceVysledku	Adam, Vojtěch Hubálek, Jaromír Hynek, David Kizek, René Ryvolová, Markéta Trnková, Libuše Šobrová, Pavlína
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2012-01-01 (xsd:date)
number of pages	3 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Mendelova zemědělská a lesnická univerzita v Brně
https://schema.org/isbn	9788073756185
http://localhost/t...ganizacniJednotka	14310

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