About: Magnetic Hydrophilic Poly(2-Hydroxyethyl Methacrylate-co-Glycidyl Methacrylate) Microspheres for DNA Isolation from Faeces

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Magnetic non-porous hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate)-P(HEMA-co-GMA) microspheres containing carboxyl groups were used for DNA isolation from mouse faeces spiked by a probiotic Lactobacillus gasseri K7 strain. The quality of isolated DNA and the presence of target DNA were verified by PCR and real time PCR using primers specific for Lactobacillus genus or primers targeting gassericin A gene of the Lactobacillus gasseri K7 strain. For comparison, two other DNA extraction procedures were used. It was shown that DNA extracted by carboxylcoated P(HEMA-co-GMA) microspheres were sufficient for the amplification of target DNA using PCR and real-time PCR. Magnetic non-porous hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate)-P(HEMA-co-GMA) microspheres containing carboxyl groups were used for DNA isolation from mouse faeces spiked by a probiotic Lactobacillus gasseri K7 strain. The quality of isolated DNA and the presence of target DNA were verified by PCR and real time PCR using primers specific for Lactobacillus genus or primers targeting gassericin A gene of the Lactobacillus gasseri K7 strain. For comparison, two other DNA extraction procedures were used. It was shown that DNA extracted by carboxylcoated P(HEMA-co-GMA) microspheres were sufficient for the amplification of target DNA using PCR and real-time PCR. (en)
Title	Magnetic Hydrophilic Poly(2-Hydroxyethyl Methacrylate-co-Glycidyl Methacrylate) Microspheres for DNA Isolation from Faeces Magnetic Hydrophilic Poly(2-Hydroxyethyl Methacrylate-co-Glycidyl Methacrylate) Microspheres for DNA Isolation from Faeces (en)
skos:prefLabel	Magnetic Hydrophilic Poly(2-Hydroxyethyl Methacrylate-co-Glycidyl Methacrylate) Microspheres for DNA Isolation from Faeces Magnetic Hydrophilic Poly(2-Hydroxyethyl Methacrylate-co-Glycidyl Methacrylate) Microspheres for DNA Isolation from Faeces (en)
skos:notation	RIV/00216224:14310/12:00059333!RIV13-MSM-14310___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(2B06053), S, Z(AV0Z40500505), Z(MSM0021622415)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Španová, Alena Rittich, Bohuslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	147758
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/12:00059333
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	DNA isolation; magnetic microspheres; mouse faeces; P(HEMA-co-GMA); PCR (en)
http://linked.open.../riv/klicoveSlovo	DNA isolation PCR magnetic microspheres P(HEMA-co-GMA) mouse faeces
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[2CF6D9DB8E54]
http://linked.open...i/riv/nazevZdroje	Molecular Crystals and Liquid Crystals
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	New methods for the characterization and identification of probiotic bacterial strains suitable for functional foods.
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	555
http://linked.open...iv/tvurceVysledku	Horák, Daniel Rittich, Bohuslav Trachtová, Štěpánka Španová, Alena Matijašic, Bojana Bogovic Obermajer, Tanja Rogelj, Irena
http://linked.open...ain/vavai/riv/wos	000302300400027
http://linked.open...n/vavai/riv/zamer	Progresivní makromolekulární materiály a supramolekulární systémy: syntéza a studium vlastností, jevů a možností využití pro speciální aplikace a moderní technologie Molecular basis of cell and tissue regulations
issn	1542-1406
number of pages	8 (xsd:int)
http://bibframe.org/vocab/doi	10.1080/15421406.2012.635554
http://localhost/t...ganizacniJednotka	14310

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