About: Biotinylation of quantum dots for application in fluoroimmunoassays with biotin-avidin amplification     Goto   Sponge   NotDistinct   Permalink

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  • A competitive microplate fluoroimmunoassay was developed for the determination of human serum albumin in urine. It is based on the use of biotinylated CdTe quantum dots (QDs) whose synthesis is optimised in terms of storage stability, purification, and signal-to-noise ratio. The bioconjugated QDs were characterised by gel chromatography and gel electrophoresis. Storage stability and quantum yield were investigated. The excitation/emission wavelengths are 360/620 nm. The immunoassay of human serum albumin in urine has a working range from 1.7 to 10 ug/mL, and the limit of detection is 1.0 ug/mL.
  • A competitive microplate fluoroimmunoassay was developed for the determination of human serum albumin in urine. It is based on the use of biotinylated CdTe quantum dots (QDs) whose synthesis is optimised in terms of storage stability, purification, and signal-to-noise ratio. The bioconjugated QDs were characterised by gel chromatography and gel electrophoresis. Storage stability and quantum yield were investigated. The excitation/emission wavelengths are 360/620 nm. The immunoassay of human serum albumin in urine has a working range from 1.7 to 10 ug/mL, and the limit of detection is 1.0 ug/mL. (en)
Title
  • Biotinylation of quantum dots for application in fluoroimmunoassays with biotin-avidin amplification
  • Biotinylation of quantum dots for application in fluoroimmunoassays with biotin-avidin amplification (en)
skos:prefLabel
  • Biotinylation of quantum dots for application in fluoroimmunoassays with biotin-avidin amplification
  • Biotinylation of quantum dots for application in fluoroimmunoassays with biotin-avidin amplification (en)
skos:notation
  • RIV/00216224:14310/12:00059178!RIV13-MSM-14310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(LC06030), S, Z(MSM0021622413)
http://linked.open...iv/cisloPeriodika
  • 3-4
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 125198
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/12:00059178
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • biotin; avidin; albumin; bioconjugation (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • AT - Rakouská republika
http://linked.open...ontrolniKodProRIV
  • [62ADF33A6B0D]
http://linked.open...i/riv/nazevZdroje
  • Microchimica Acta
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 176
http://linked.open...iv/tvurceVysledku
  • Skládal, Petr
  • Bouchal, Pavel
  • Hlaváček, Antonín
http://linked.open...ain/vavai/riv/wos
  • 000299921400004
http://linked.open...n/vavai/riv/zamer
issn
  • 0026-3672
number of pages
http://bibframe.org/vocab/doi
  • 10.1007/s00604-011-0729-6
http://localhost/t...ganizacniJednotka
  • 14310
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