About: Unwinding of synthetic replication and recombination substrates by Srs2

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About: Unwinding of synthetic replication and recombination substrates by Srs2 Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The budding yeast Srs2 protein possesses 3 to 5 DNA helicase activity and channels untimely recombination to post-replication repair by removing Rad51 from ssDNA. However, it also promotes recombination via a synthesis-dependent strand-annealing pathway (SDSA). Furthermore, at the replication fork, Srs2 is required for fork progression and prevents the instability of trinucleotide repeats. To better understand the multiple roles of the Srs2 helicase during these processes, we analysed the ability of Srs2 to bind and unwind various DNA substrates that mimic structures present during DNA replication and recombination. While leading or lagging strands were efficiently unwound, the presence of ssDNA binding protein RPA presented an obstacle for Srs2 translocation. We also tested the preferred directionality of unwinding of various substrates and studied the effect of Rad51 and Mre11 proteins on Srs2 helicase activity. The budding yeast Srs2 protein possesses 3 to 5 DNA helicase activity and channels untimely recombination to post-replication repair by removing Rad51 from ssDNA. However, it also promotes recombination via a synthesis-dependent strand-annealing pathway (SDSA). Furthermore, at the replication fork, Srs2 is required for fork progression and prevents the instability of trinucleotide repeats. To better understand the multiple roles of the Srs2 helicase during these processes, we analysed the ability of Srs2 to bind and unwind various DNA substrates that mimic structures present during DNA replication and recombination. While leading or lagging strands were efficiently unwound, the presence of ssDNA binding protein RPA presented an obstacle for Srs2 translocation. We also tested the preferred directionality of unwinding of various substrates and studied the effect of Rad51 and Mre11 proteins on Srs2 helicase activity. (en)
Title	Unwinding of synthetic replication and recombination substrates by Srs2 Unwinding of synthetic replication and recombination substrates by Srs2 (en)
skos:prefLabel	Unwinding of synthetic replication and recombination substrates by Srs2 Unwinding of synthetic replication and recombination substrates by Srs2 (en)
skos:notation	RIV/00216224:14310/12:00057534!RIV13-GA0-14310___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(ED1.100/02/0123), P(GA301/09/1917), P(GAP207/12/2323), P(GD203/09/H046), P(ME10048)
http://linked.open...iv/cisloPeriodika	10
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Marini Palomeque, María Victoria Krejčí, Lumír
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	176091
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/12:00057534
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	DNA repair; Recombination; Srs2; Replication; Helicase (en)
http://linked.open.../riv/klicoveSlovo	Recombination DNA repair Replication Srs2 Helicase
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[B6DA39D06FE1]
http://linked.open...i/riv/nazevZdroje	DNA Repair
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Biochemistry on the crossroad from in silico to in vitro Endonuclease and translocase activity in type I restriction-modification complexes St. Anne´s University Hospital Brno - International Clinical Research Center (FNUSA-ICRC) Resolution of replication-recombination DNA intermediates and its role in genomic instability The role of post-translational modification in DNA repair and recombination: Meiosis, SUMOylation and Zip3 protein
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	11
http://linked.open...iv/tvurceVysledku	Krejčí, Lumír Marini Palomeque, María Victoria
http://linked.open...ain/vavai/riv/wos	000310761100002
issn	1568-7864
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.dnarep.2012.05.007
http://localhost/t...ganizacniJednotka	14310

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