About: Measurements of genomic GC content in plant genomes with flow cytometry: a test for reliability

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Knowledge of phylogenetic pattern and biological relevance of the base composition of large eukaryotic genomes (including plants) is poor. With the use of flow cytometry (FCM), the available data on the GC content in plants have nearly doubled in the last decade. However, skepticism exists concerning the reliability of the method because of uncertainty in some input parameters. Here, we tested the reliability of FCM for estimating the GC content by comparison with the biochemical method of DNA melting temperature analysis (TMA). We conducted measurements in 14 plant species with a maximum currently known GC content range (33.6–47.5%; FCM). We also compared the estimations of the GC content by FCM with genomic sequences in 11 Oryza species. FCM and TMA data exhibited a high degree of correspondence that remained stable over the relatively wide range of binding lengths (3.39–4.09) assumed for the base-specific dye used. Knowledge of phylogenetic pattern and biological relevance of the base composition of large eukaryotic genomes (including plants) is poor. With the use of flow cytometry (FCM), the available data on the GC content in plants have nearly doubled in the last decade. However, skepticism exists concerning the reliability of the method because of uncertainty in some input parameters. Here, we tested the reliability of FCM for estimating the GC content by comparison with the biochemical method of DNA melting temperature analysis (TMA). We conducted measurements in 14 plant species with a maximum currently known GC content range (33.6–47.5%; FCM). We also compared the estimations of the GC content by FCM with genomic sequences in 11 Oryza species. FCM and TMA data exhibited a high degree of correspondence that remained stable over the relatively wide range of binding lengths (3.39–4.09) assumed for the base-specific dye used. (en)
Title	Measurements of genomic GC content in plant genomes with flow cytometry: a test for reliability Measurements of genomic GC content in plant genomes with flow cytometry: a test for reliability (en)
skos:prefLabel	Measurements of genomic GC content in plant genomes with flow cytometry: a test for reliability Measurements of genomic GC content in plant genomes with flow cytometry: a test for reliability (en)
skos:notation	RIV/00216224:14310/12:00057231!RIV13-GA0-14310___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(GA206/09/1405), P(GAP506/10/1363), P(GAP506/11/0890), P(GP206/08/P222), P(LC06073), S, Z(MSM0021622416)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Bureš, Petr Horová, Lucie Šmerda, Jakub
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	149028
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/12:00057231
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	DNA base composition; flow cytometry; GC content; plant genomes; temperature DNA melting (en)
http://linked.open.../riv/klicoveSlovo	flow cytometry GC content plant genomes temperature DNA melting DNA base composition
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[663D91AE53B4]
http://linked.open...i/riv/nazevZdroje	New Phytologist
http://linked.open...in/vavai/riv/obor	EF
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Evolution of DNA base composition in land plants GC content and genome evolution of grasses Genome size and karyotype evolution in Cyperaceae Biodiversity Research Center Phylogeny of subtribe Hieraciinae (Asteraceae) – a model example of contrasting evolutionary strategies in closely related lineages
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	193
http://linked.open...iv/tvurceVysledku	Bureš, Petr Šmarda, Petr Horová, Lucie Šmerda, Jakub
http://linked.open...ain/vavai/riv/wos	000298591800024
http://linked.open...n/vavai/riv/zamer	Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time
issn	0028-646X
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1111/j.1469-8137.2011.03942.x
http://localhost/t...ganizacniJednotka	14310

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