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  • Knowledge of phylogenetic pattern and biological relevance of the base composition of large eukaryotic genomes (including plants) is poor. With the use of flow cytometry (FCM), the available data on the GC content in plants have nearly doubled in the last decade. However, skepticism exists concerning the reliability of the method because of uncertainty in some input parameters. Here, we tested the reliability of FCM for estimating the GC content by comparison with the biochemical method of DNA melting temperature analysis (TMA). We conducted measurements in 14 plant species with a maximum currently known GC content range (33.6–47.5%; FCM). We also compared the estimations of the GC content by FCM with genomic sequences in 11 Oryza species. FCM and TMA data exhibited a high degree of correspondence that remained stable over the relatively wide range of binding lengths (3.39–4.09) assumed for the base-specific dye used.
  • Knowledge of phylogenetic pattern and biological relevance of the base composition of large eukaryotic genomes (including plants) is poor. With the use of flow cytometry (FCM), the available data on the GC content in plants have nearly doubled in the last decade. However, skepticism exists concerning the reliability of the method because of uncertainty in some input parameters. Here, we tested the reliability of FCM for estimating the GC content by comparison with the biochemical method of DNA melting temperature analysis (TMA). We conducted measurements in 14 plant species with a maximum currently known GC content range (33.6–47.5%; FCM). We also compared the estimations of the GC content by FCM with genomic sequences in 11 Oryza species. FCM and TMA data exhibited a high degree of correspondence that remained stable over the relatively wide range of binding lengths (3.39–4.09) assumed for the base-specific dye used. (en)
Title
  • Measurements of genomic GC content in plant genomes with flow cytometry: a test for reliability
  • Measurements of genomic GC content in plant genomes with flow cytometry: a test for reliability (en)
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  • Measurements of genomic GC content in plant genomes with flow cytometry: a test for reliability
  • Measurements of genomic GC content in plant genomes with flow cytometry: a test for reliability (en)
skos:notation
  • RIV/00216224:14310/12:00057231!RIV13-GA0-14310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA206/09/1405), P(GAP506/10/1363), P(GAP506/11/0890), P(GP206/08/P222), P(LC06073), S, Z(MSM0021622416)
http://linked.open...iv/cisloPeriodika
  • 2
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http://linked.open...aciTvurceVysledku
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  • 149028
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  • RIV/00216224:14310/12:00057231
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  • DNA base composition; flow cytometry; GC content; plant genomes; temperature DNA melting (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [663D91AE53B4]
http://linked.open...i/riv/nazevZdroje
  • New Phytologist
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http://linked.open...v/svazekPeriodika
  • 193
http://linked.open...iv/tvurceVysledku
  • Bureš, Petr
  • Šmarda, Petr
  • Horová, Lucie
  • Šmerda, Jakub
http://linked.open...ain/vavai/riv/wos
  • 000298591800024
http://linked.open...n/vavai/riv/zamer
issn
  • 0028-646X
number of pages
http://bibframe.org/vocab/doi
  • 10.1111/j.1469-8137.2011.03942.x
http://localhost/t...ganizacniJednotka
  • 14310
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