About: Structural and Functional Characterization of the Wnt Inhibitor APC Membrane Recruitment 1 (Amer1)

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Structural and Functional Characterization of the Wnt Inhibitor APC Membrane Recruitment 1 (Amer1) Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Amer1/WTX binds to the tumor suppressor adenomatous polyposis coli and acts as an inhibitor of Wnt signaling by inducing beta catenin degradation. We show here that Amer1 directly interacts with the armadillo repeats of beta catenin via a domain consisting of repeated arginine-glutamic acid-alanine (REA) motifs, and that Amer1 assembles the beta catenin destruction complex at the plasma membrane by recruiting beta catenin, adenomatous polyposis coli, and Axin/Conductin. Deletion or specific mutations of the membrane binding domain of Amer1 abolish its membrane localization and abrogate negative control of Wnt signaling, which can be restored by artificial targeting of Amer1 to the plasma membrane. In line, a natural splice variant of Amer1 lacking the plasma membrane localization domain is deficient for Wnt inhibition. Knockdown of Amer1 leads to the activation of Wnt target genes, preferentially in dense compared with sparse cell cultures, suggesting that Amer1 function is regulated by cell contacts. Amer1/WTX binds to the tumor suppressor adenomatous polyposis coli and acts as an inhibitor of Wnt signaling by inducing beta catenin degradation. We show here that Amer1 directly interacts with the armadillo repeats of beta catenin via a domain consisting of repeated arginine-glutamic acid-alanine (REA) motifs, and that Amer1 assembles the beta catenin destruction complex at the plasma membrane by recruiting beta catenin, adenomatous polyposis coli, and Axin/Conductin. Deletion or specific mutations of the membrane binding domain of Amer1 abolish its membrane localization and abrogate negative control of Wnt signaling, which can be restored by artificial targeting of Amer1 to the plasma membrane. In line, a natural splice variant of Amer1 lacking the plasma membrane localization domain is deficient for Wnt inhibition. Knockdown of Amer1 leads to the activation of Wnt target genes, preferentially in dense compared with sparse cell cultures, suggesting that Amer1 function is regulated by cell contacts. (en)
Title	Structural and Functional Characterization of the Wnt Inhibitor APC Membrane Recruitment 1 (Amer1) Structural and Functional Characterization of the Wnt Inhibitor APC Membrane Recruitment 1 (Amer1) (en)
skos:prefLabel	Structural and Functional Characterization of the Wnt Inhibitor APC Membrane Recruitment 1 (Amer1) Structural and Functional Characterization of the Wnt Inhibitor APC Membrane Recruitment 1 (Amer1) (en)
skos:notation	RIV/00216224:14310/11:00054246!RIV12-MSM-14310___
http://linked.open...avai/riv/aktivita	S Z
http://linked.open...avai/riv/aktivity	S, Z(MSM0021622430)
http://linked.open...iv/cisloPeriodika	22
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Kříž, Vítězslav Bryja, Vítězslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	232722
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/11:00054246
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Amer1 wtx REA motifs beta catenin wnt signaling Axin (en)
http://linked.open.../riv/klicoveSlovo	Amer1 wtx REA motifs beta catenin wnt signaling Axin
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[E97F125C654A]
http://linked.open...i/riv/nazevZdroje	JOURNAL OF BIOLOGICAL CHEMISTRY
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	286
http://linked.open...iv/tvurceVysledku	Behrens, J. Bryja, Vítězslav Kříž, Vítězslav Tanneberger, K. Pfister, A. Schambony, A.
http://linked.open...ain/vavai/riv/wos	000291027700003
http://linked.open...n/vavai/riv/zamer	Functional and molecular characteristics of cancer and normal stem cells - identification of targets for novel therapeutics and therapeutic strategies
issn	0021-9258
number of pages	11 (xsd:int)
http://bibframe.org/vocab/doi	10.1074/jbc.M111.224881
http://localhost/t...ganizacniJednotka	14310

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 49 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software