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  • A comparative examination of reduced methyl [MV](+) and benzyl [BV](+) viologens (as artificial electron donors for quantitative estimation of the respiratory periplasmic (Nap) and membrane-embedded (Nar) nitrate reductases) using a newly constructed nap mutant strain of Paracocccus denitrificans was done. The activity with [MV](+) was high in whole-cell assays, confirming that this compound donates electrons to Nar. Initial rates of the more lipophilic [BV](+) were considerably lower, which was interpreted to be caused by an inhibition of the active transport of nitrate into the cells. Anionophoric activity of [BV](+) was detectable but too low to effectively circumvent the inhibition of nitrate transporter.
  • A comparative examination of reduced methyl [MV](+) and benzyl [BV](+) viologens (as artificial electron donors for quantitative estimation of the respiratory periplasmic (Nap) and membrane-embedded (Nar) nitrate reductases) using a newly constructed nap mutant strain of Paracocccus denitrificans was done. The activity with [MV](+) was high in whole-cell assays, confirming that this compound donates electrons to Nar. Initial rates of the more lipophilic [BV](+) were considerably lower, which was interpreted to be caused by an inhibition of the active transport of nitrate into the cells. Anionophoric activity of [BV](+) was detectable but too low to effectively circumvent the inhibition of nitrate transporter. (en)
Title
  • Nitrate reductase whole-cell assay: side effects associated with the use of benzyl viologen
  • Nitrate reductase whole-cell assay: side effects associated with the use of benzyl viologen (en)
skos:prefLabel
  • Nitrate reductase whole-cell assay: side effects associated with the use of benzyl viologen
  • Nitrate reductase whole-cell assay: side effects associated with the use of benzyl viologen (en)
skos:notation
  • RIV/00216224:14310/11:00052814!RIV12-MSM-14310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM0021622413)
http://linked.open...iv/cisloPeriodika
  • 1
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 216058
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/11:00052814
http://linked.open...riv/jazykVysledku
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  • nitrate reductase; artificial electron donor; nitrate transport; inhibition (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [A883146C91D1]
http://linked.open...i/riv/nazevZdroje
  • Folia Microbiologica
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http://linked.open...ichTvurcuVysledku
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http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 56
http://linked.open...iv/tvurceVysledku
  • Kučera, Igor
  • Illichmanová, Adéla
  • Janů, Miroslava
  • Sedláček, Vojtěch
http://linked.open...ain/vavai/riv/wos
  • 000290451500012
http://linked.open...n/vavai/riv/zamer
issn
  • 0015-5632
number of pages
http://bibframe.org/vocab/doi
  • 10.1007/s12223-011-0004-6
http://localhost/t...ganizacniJednotka
  • 14310
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