About: Voltametrická studie cytosinových oligonukleotidů v pufrovaném a nepufrovaném prostředí

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The work deals with the electrochemical study of three short homo–deoxyoligonucleotides (homo–ODNs) containing cytosine (C). Using linear sweep voltammetry (LSV) and elimination voltammetry with linear scan (EVLS) the effect of pH and the number of C in the ODN chain on reduction signals of dC3, dC6, and dC9 in buffered and non-buffered solutions was investigated. Reduction C peaks, recorded at mercury electrode in the potential range from –1.1 V to –1.7 V vs. Ag/AgCl/3M KCl, are strongly dependent not only on pH but also on the composition of solutions. With the addition of buffer (acetate-phosphate buffer) to 0.1 M NaCl the reduction peaks increase and move to more positive potentials. In non-buffered solutions changes of voltammetric responses of all ODNs in time were observed. The biggest changes were recorded in the case of dC6. The adsorption or surface kinetics of ODNs was confirmed by EVLS and electrochemical behaviour of ODNs studied was discussed in the context of the i-motif formation. The work deals with the electrochemical study of three short homo–deoxyoligonucleotides (homo–ODNs) containing cytosine (C). Using linear sweep voltammetry (LSV) and elimination voltammetry with linear scan (EVLS) the effect of pH and the number of C in the ODN chain on reduction signals of dC3, dC6, and dC9 in buffered and non-buffered solutions was investigated. Reduction C peaks, recorded at mercury electrode in the potential range from –1.1 V to –1.7 V vs. Ag/AgCl/3M KCl, are strongly dependent not only on pH but also on the composition of solutions. With the addition of buffer (acetate-phosphate buffer) to 0.1 M NaCl the reduction peaks increase and move to more positive potentials. In non-buffered solutions changes of voltammetric responses of all ODNs in time were observed. The biggest changes were recorded in the case of dC6. The adsorption or surface kinetics of ODNs was confirmed by EVLS and electrochemical behaviour of ODNs studied was discussed in the context of the i-motif formation. (en)
Title	Voltametrická studie cytosinových oligonukleotidů v pufrovaném a nepufrovaném prostředí Voltametrická studie cytosinových oligonukleotidů v pufrovaném a nepufrovaném prostředí (en)
skos:prefLabel	Voltametrická studie cytosinových oligonukleotidů v pufrovaném a nepufrovaném prostředí Voltametrická studie cytosinových oligonukleotidů v pufrovaném a nepufrovaném prostředí (en)
skos:notation	RIV/00216224:14310/11:00052416!RIV12-MSM-14310___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(LC06035), S, Z(MSM0021622412)
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Holubová, Sylvie Balcarová, Zdeňka Trnková, Libuše
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	239501
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/11:00052416
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cytosine; oligonucleotides; cyclic voltammetry; elimination voltammetry (en)
http://linked.open.../riv/klicoveSlovo	cyclic voltammetry cytosine oligonucleotides elimination voltammetry
http://linked.open...ontrolniKodProRIV	[73BE17E20B93]
http://linked.open...v/mistoKonaniAkce	Jetřichovice
http://linked.open...i/riv/mistoVydani	Ústí nad Labem
http://linked.open...i/riv/nazevZdroje	XXXI. MODERNÍ ELEKTROCHEMICKÉ METODY
http://linked.open...in/vavai/riv/obor	CF
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine.
http://linked.open...UplatneniVysledku	2011
http://linked.open...iv/tvurceVysledku	Balcarová, Zdeňka Holubová, Sylvie Trnková, Libuše
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2011-01-01 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)
number of pages	4 (xsd:int)
http://purl.org/ne...btex#hasPublisher	BEST Servis
https://schema.org/isbn	978-80-254-9634-3
http://localhost/t...ganizacniJednotka	14310

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