About: Measurements of DNA base composition with flow cytometry and DNA temperature melting

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Base composition (expressed most frequently as GC content) is an important classification criterion in microbial research. This might be also the case of plants, however, the data on GC contents in plants are still very poor and some skepticism still exist on the reliability of older estimates as well as on the accuracy of recently cumulating data from flow cytometry (FCM) experiments. FCM enables to measure GC content by comparing results with base specific and base unspecific dyes, most frequently unspecific PI and AT-specific DAPI (Barow et Meister, 2002; Bureš et al., 2004; Šmarda et al., 2008). This measurements are critically dependent on the knowledge of binding length of the used base specific dye (i.e., the number of consecutive pairs of the same type enabling binding of a dye molecule), which is, however, mostly uncertain for many dyes and may vary with specific base patterning in a natural DNA. Base composition (expressed most frequently as GC content) is an important classification criterion in microbial research. This might be also the case of plants, however, the data on GC contents in plants are still very poor and some skepticism still exist on the reliability of older estimates as well as on the accuracy of recently cumulating data from flow cytometry (FCM) experiments. FCM enables to measure GC content by comparing results with base specific and base unspecific dyes, most frequently unspecific PI and AT-specific DAPI (Barow et Meister, 2002; Bureš et al., 2004; Šmarda et al., 2008). This measurements are critically dependent on the knowledge of binding length of the used base specific dye (i.e., the number of consecutive pairs of the same type enabling binding of a dye molecule), which is, however, mostly uncertain for many dyes and may vary with specific base patterning in a natural DNA. (en)
Title	Measurements of DNA base composition with flow cytometry and DNA temperature melting Measurements of DNA base composition with flow cytometry and DNA temperature melting (en)
skos:prefLabel	Measurements of DNA base composition with flow cytometry and DNA temperature melting Measurements of DNA base composition with flow cytometry and DNA temperature melting (en)
skos:notation	RIV/00216224:14310/11:00050575!RIV12-GA0-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA206/09/1405), P(GAP506/11/0890), P(LC06073), Z(MSM0021622416)
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Šmarda, Petr Bureš, Petr Horová, Lucie Šmerda, Jakub
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	211171
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/11:00050575
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	DNA base composition; flow cytometry; DNA temperature melting (en)
http://linked.open.../riv/klicoveSlovo	flow cytometry DNA base composition DNA temperature melting
http://linked.open...ontrolniKodProRIV	[7982ABE138DE]
http://linked.open...in/vavai/riv/obor	EF
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Evolution of DNA base composition in land plants Genome size and karyotype evolution in Cyperaceae Biodiversity Research Center
http://linked.open...UplatneniVysledku	2011
http://linked.open...iv/tvurceVysledku	Bureš, Petr Šmarda, Petr Horová, Lucie Šmerda, Jakub
http://linked.open...n/vavai/riv/zamer	Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time
http://localhost/t...ganizacniJednotka	14310

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