About: CD133 expression and identification of CD133/nestin positive cells in rhabdomyosarcomas and rhabdomyosarcoma cell lines

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About: CD133 expression and identification of CD133/nestin positive cells in rhabdomyosarcomas and rhabdomyosarcoma cell lines Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Co-expression of CD133, cell surface glycoprotein, and nestin, an intermediate filament protein, was determined to be a marker of neural stem cells and of cancer stem cells in neurogenic tumors. Methods: We examined the expression of CD133 and nestin in ten tumor tissue samples taken from patients with rhabdomyosarcomas and in five rhabdomyosarcoma cell lines. Immunohistochemistry and immunofluorescence were used to examine FFPE tumor tissue samples. Cell lines were analyzed by immunofluorescence, immunoblotting, flow cytometry, and RT-PCR. Functional assays (clonogenic in vitro assay and tumorigenic in vivo assay) were also performed using these cell lines. Results: CD133 and nestin were detected in all 10 tumor tissue samples and in all 5 cell lines; however, the frequency of CD133+, Nes+, and CD133+/Nes+ cells, as well as the intensity of fluorescence varied in individual samples or cell lines. The expression of CD133 and nestin was subsequently confirmed in all cell lines by immunoblotting. Co-expression of CD133, cell surface glycoprotein, and nestin, an intermediate filament protein, was determined to be a marker of neural stem cells and of cancer stem cells in neurogenic tumors. Methods: We examined the expression of CD133 and nestin in ten tumor tissue samples taken from patients with rhabdomyosarcomas and in five rhabdomyosarcoma cell lines. Immunohistochemistry and immunofluorescence were used to examine FFPE tumor tissue samples. Cell lines were analyzed by immunofluorescence, immunoblotting, flow cytometry, and RT-PCR. Functional assays (clonogenic in vitro assay and tumorigenic in vivo assay) were also performed using these cell lines. Results: CD133 and nestin were detected in all 10 tumor tissue samples and in all 5 cell lines; however, the frequency of CD133+, Nes+, and CD133+/Nes+ cells, as well as the intensity of fluorescence varied in individual samples or cell lines. The expression of CD133 and nestin was subsequently confirmed in all cell lines by immunoblotting. (en)
Title	CD133 expression and identification of CD133/nestin positive cells in rhabdomyosarcomas and rhabdomyosarcoma cell lines CD133 expression and identification of CD133/nestin positive cells in rhabdomyosarcomas and rhabdomyosarcoma cell lines (en)
skos:prefLabel	CD133 expression and identification of CD133/nestin positive cells in rhabdomyosarcomas and rhabdomyosarcoma cell lines CD133 expression and identification of CD133/nestin positive cells in rhabdomyosarcomas and rhabdomyosarcoma cell lines (en)
skos:notation	RIV/00216224:14310/11:00050456!RIV12-MZ0-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GD204/08/H054), P(NR9125), Z(MSM0021622415), Z(MSM0021622430)
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Veselská, Renata Štěrba, Jaroslav Zitterbart, Karel Neradil, Jakub Šána, Jiří Chlapek, Petr Škoda, Jan Zambo, Iva Hampl, Aleš Hermanová, Markéta Múdry, Peter
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	189343
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/11:00050456
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Rhabdomyosarcoma; CD133; nestin; cancer stem cells; stem cell related markers (en)
http://linked.open.../riv/klicoveSlovo	nestin CD133 Rhabdomyosarcoma cancer stem cells stem cell related markers
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[A634E5A33CD3]
http://linked.open...i/riv/nazevZdroje	Analytical Cellular Pathology-Cellular Oncology
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	11 (xsd:int)
http://linked.open...cetTvurcuVysledku	12 (xsd:int)
http://linked.open...vavai/riv/projekt	Molecular mechanisms of the cell proliferation and differentiation Importance of molecular cytogenetic markers for diagnosis and prediction of embryonal childhood tumors.
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	34
http://linked.open...iv/tvurceVysledku	Chlapek, Petr Hermanová, Markéta Hampl, Aleš Múdry, Peter Neradil, Jakub Vasikova, Alzbeta Veselská, Renata Zitterbart, Karel Zambo, Iva Škoda, Jan Štěrba, Jaroslav Šána, Jiří
http://linked.open...ain/vavai/riv/wos	000298742900002
http://linked.open...n/vavai/riv/zamer	Molecular basis of cell and tissue regulations Functional and molecular characteristics of cancer and normal stem cells - identification of targets for novel therapeutics and therapeutic strategies
issn	2210-7177
number of pages	16 (xsd:int)
http://bibframe.org/vocab/doi	10.3233/ACP-2011-0018
http://localhost/t...ganizacniJednotka	14310

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