About: Cloning, expression and structure determination of SH3b cell wall binding domain of bacteriophage 812 endolysin

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About: Cloning, expression and structure determination of SH3b cell wall binding domain of bacteriophage 812 endolysin Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In this study we focused on endolysin of staphylococcal bacteriophage 812, a member of unclassified SPO1-like viruses from family Myoviridae. The endolysin of phage 812 includes three domains: two catalytic domains and the SH3b-domain that is probably a cell wall targeting domain, which could be responsible for binding of endolysin to peptidoglycan. The gene sequence for the C-terminal SH3b domain was cloned in pET28 vector and expressed as a soluble protein in E. coli BL21 (DE3) to determine the 3D structure of the protein. A native variant of the SH3b protein was purified without the his-tag. Subsequently, three variants of this protein have been prepared: (i) non-labeled, (ii) single-labeled (15N) and (iii) double-labeled (13C, 15N) domain. The protein was purified to homogeneity using ammonium sulphate precipitation, anion exchange chromatography, cation exchange chromatography, and gel filtration. In this study we focused on endolysin of staphylococcal bacteriophage 812, a member of unclassified SPO1-like viruses from family Myoviridae. The endolysin of phage 812 includes three domains: two catalytic domains and the SH3b-domain that is probably a cell wall targeting domain, which could be responsible for binding of endolysin to peptidoglycan. The gene sequence for the C-terminal SH3b domain was cloned in pET28 vector and expressed as a soluble protein in E. coli BL21 (DE3) to determine the 3D structure of the protein. A native variant of the SH3b protein was purified without the his-tag. Subsequently, three variants of this protein have been prepared: (i) non-labeled, (ii) single-labeled (15N) and (iii) double-labeled (13C, 15N) domain. The protein was purified to homogeneity using ammonium sulphate precipitation, anion exchange chromatography, cation exchange chromatography, and gel filtration. (en)
Title	Cloning, expression and structure determination of SH3b cell wall binding domain of bacteriophage 812 endolysin Cloning, expression and structure determination of SH3b cell wall binding domain of bacteriophage 812 endolysin (en)
skos:prefLabel	Cloning, expression and structure determination of SH3b cell wall binding domain of bacteriophage 812 endolysin Cloning, expression and structure determination of SH3b cell wall binding domain of bacteriophage 812 endolysin (en)
skos:notation	RIV/00216224:14310/11:00050057!RIV12-TA0-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA310/09/0459), P(GAP206/11/0758), P(GP204/02/D099), P(TA01010405), Z(MSM0021622415)
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Janda, Lubomír Dopitová, Radka Benešík, Martin Doškař, Jiří Růžičková, Vladislava Žídek, Lukáš Nováček, Jiří Pantůček, Roman
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	190638
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/11:00050057
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	bacteriophage therapy; Staphylococcus aureus; bacteriophage endolysin; SH3b domain (en)
http://linked.open.../riv/klicoveSlovo	SH3b domain Staphylococcus aureus bacteriophage endolysin bacteriophage therapy
http://linked.open...ontrolniKodProRIV	[AA634D126475]
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	8 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...vavai/riv/projekt	Role of bacteriophages in horizontal transfer of virulence and drug resistance genes in Staphylococcus aureus Development of high-resolution methodology for NMR studies of disordered proteins with highly degenerated resonance frequencies Database of the ribotype profiles of bacterial type strains Research of broad host-range mutants of staphylococcal bacteriophages
http://linked.open...UplatneniVysledku	2011
http://linked.open...iv/tvurceVysledku	Benešík, Martin Dopitová, Radka Doškař, Jiří Janda, Lubomír Moša, Marek Nováček, Jiří Pantůček, Roman Růžičková, Vladislava Žídek, Lukáš
http://linked.open...n/vavai/riv/zamer	Molecular basis of cell and tissue regulations
http://localhost/t...ganizacniJednotka	14310

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