About: The effects of wedelolactone on cancer cells depend on its redox state

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About: The effects of wedelolactone on cancer cells depend on its redox state Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The aim of this study was to further characterize the mechanism how wedelolactone affects topoisomerase IIa and cancer cells. Using electrophoretic mobility shift assay we found that wedelolactone inhibited binding of topoisomerase IIa to supercoiled plasmid DNA. The inhibitory effect of wedelolactone on the topoisomerase IIa was reversed by excess of enzyme but not DNA suggesting that wedelolactone exerted its inhibitory effect by interaction with the topoisomerase II protein. The in vitro inhibitory effect of wedelolactone on the topoisomerase IIa activity was redox-dependent as it diminished in the presence of reducing agents, such as DTT, glutathione, N-acetylcysteine and L-ascorbic acid. Similarly, cytotoxicity of wedelolactone in breast cancer MDA-MB-231 cells was inhibited by N-acetylcysteine but enhanced by buthionine sulfoximine, an inhibitor of glutathione synthesis. Finally, we found that wedelolactone can be oxidized in the presence of copper ions to semiquinone/quinone radicals. The aim of this study was to further characterize the mechanism how wedelolactone affects topoisomerase IIa and cancer cells. Using electrophoretic mobility shift assay we found that wedelolactone inhibited binding of topoisomerase IIa to supercoiled plasmid DNA. The inhibitory effect of wedelolactone on the topoisomerase IIa was reversed by excess of enzyme but not DNA suggesting that wedelolactone exerted its inhibitory effect by interaction with the topoisomerase II protein. The in vitro inhibitory effect of wedelolactone on the topoisomerase IIa activity was redox-dependent as it diminished in the presence of reducing agents, such as DTT, glutathione, N-acetylcysteine and L-ascorbic acid. Similarly, cytotoxicity of wedelolactone in breast cancer MDA-MB-231 cells was inhibited by N-acetylcysteine but enhanced by buthionine sulfoximine, an inhibitor of glutathione synthesis. Finally, we found that wedelolactone can be oxidized in the presence of copper ions to semiquinone/quinone radicals. (en)
Title	The effects of wedelolactone on cancer cells depend on its redox state The effects of wedelolactone on cancer cells depend on its redox state (en)
skos:prefLabel	The effects of wedelolactone on cancer cells depend on its redox state The effects of wedelolactone on cancer cells depend on its redox state (en)
skos:notation	RIV/00216224:14310/11:00049466!RIV12-GA0-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA301/09/1115), P(GD204/08/H054), P(IAA501630801), Z(MSM0021622415)
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Alexová, Petra Šmarda, Jan
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	196692
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/11:00049466
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	wedelolactone; topoisomerase IIa; breast cancer (en)
http://linked.open.../riv/klicoveSlovo	breast cancer topoisomerase IIa wedelolactone
http://linked.open...ontrolniKodProRIV	[80C3C4E68314]
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	The role of c-Myb and Cox-2 proteins in colon carcinogenesis Molecular mechanisms of the cell proliferation and differentiation Mechanism of procathepsin D effect on breast cancer cells
http://linked.open...UplatneniVysledku	2011
http://linked.open...iv/tvurceVysledku	Beneš, Petr Knopfová, Lucia Šmarda, Jan Alexová, Petra
http://linked.open...n/vavai/riv/zamer	Molecular basis of cell and tissue regulations
http://localhost/t...ganizacniJednotka	14310

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