About: An Electrochemical Study of DNA and RNA Short Fragments by Elimination Voltammetry

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Description	Short oligonucleotides play an important role in many biological processes including triplet repeat expansion associated with neurodegenerative diseases (fragile X syndrome, Huntington’s disease, Friedreich’s ataxia or myoclonic epilepsy) [1]. One of the most stable DNA fragments, which forms hairpin, is heptamer d(GCGAAGC) [2]. In our electrochemical study we analyzed not only this hairpin but also DNA and RNA fragments containing self-complementary nucleobases (GC) forming the stem. Both DNA and RNA analogues adsorbed on mercury electrodes provided voltammetric reduction signals of adenine and cytosine (A and C) and oxidation signals of guanine (G). We found significant differences between DNA and RNA fragments depending on their length, sequence, and pH (Fig. 1). For the resolution of A and C reduction signals and evaluation of the nature of anodic processes of the G signal in both DNA and RNA fragments, elimination voltammetry with linear scan (EVLS) was utilized [3,4]. Short oligonucleotides play an important role in many biological processes including triplet repeat expansion associated with neurodegenerative diseases (fragile X syndrome, Huntington’s disease, Friedreich’s ataxia or myoclonic epilepsy) [1]. One of the most stable DNA fragments, which forms hairpin, is heptamer d(GCGAAGC) [2]. In our electrochemical study we analyzed not only this hairpin but also DNA and RNA fragments containing self-complementary nucleobases (GC) forming the stem. Both DNA and RNA analogues adsorbed on mercury electrodes provided voltammetric reduction signals of adenine and cytosine (A and C) and oxidation signals of guanine (G). We found significant differences between DNA and RNA fragments depending on their length, sequence, and pH (Fig. 1). For the resolution of A and C reduction signals and evaluation of the nature of anodic processes of the G signal in both DNA and RNA fragments, elimination voltammetry with linear scan (EVLS) was utilized [3,4]. (en)
Title	An Electrochemical Study of DNA and RNA Short Fragments by Elimination Voltammetry An Electrochemical Study of DNA and RNA Short Fragments by Elimination Voltammetry (en)
skos:prefLabel	An Electrochemical Study of DNA and RNA Short Fragments by Elimination Voltammetry An Electrochemical Study of DNA and RNA Short Fragments by Elimination Voltammetry (en)
skos:notation	RIV/00216224:14310/10:00051671!RIV12-MSM-14310___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(LC06035), S, Z(MSM0021622412)
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Neplechová, Kamila Balcarová, Zdeňka Trnková, Libuše
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	246194
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/10:00051671
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	oligonucleotides; EVLS; heptamer; adenine; cytosine (en)
http://linked.open.../riv/klicoveSlovo	EVLS adenine cytosine oligonucleotides heptamer
http://linked.open...ontrolniKodProRIV	[290B95294F5E]
http://linked.open...in/vavai/riv/obor	CF
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine.
http://linked.open...UplatneniVysledku	2010
http://linked.open...iv/tvurceVysledku	Balcarová, Zdeňka Trnková, Libuše Jelen, František Neplechová, Kamila
http://linked.open...n/vavai/riv/zamer	Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)
http://localhost/t...ganizacniJednotka	14310

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