About: Multilocus PCR typing strategy for differentiation of Staphylococcus aureus siphoviruses reflecting their modular genome structure

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About: Multilocus PCR typing strategy for differentiation of Staphylococcus aureus siphoviruses reflecting their modular genome structure Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Given the great biological importance and high diversity of temperate Staphylococcus aureus bacteriophages, a method is needed for the description of their genomic structure. Here we have updated a multiplex PCR strategy for the complex characterization of S. aureus phages of the family Siphoviridae. Based on the comparative genomic analysis of the available phage sequences, a multilocus PCR strategy for typing the major modules of the phage genome was designed. The genomic modules were classified on the basis of the genes for integrase, anti-repressor, replication proteins, dUTPase, portal protein, tail appendices and endolysin corresponding to main functional modules. The nine PCR assays designed for the above sequences were shown to be capable to identify the bacteriophage gene pool present both in the phage and bacterial genomes and their mosaic structure. Given the great biological importance and high diversity of temperate Staphylococcus aureus bacteriophages, a method is needed for the description of their genomic structure. Here we have updated a multiplex PCR strategy for the complex characterization of S. aureus phages of the family Siphoviridae. Based on the comparative genomic analysis of the available phage sequences, a multilocus PCR strategy for typing the major modules of the phage genome was designed. The genomic modules were classified on the basis of the genes for integrase, anti-repressor, replication proteins, dUTPase, portal protein, tail appendices and endolysin corresponding to main functional modules. The nine PCR assays designed for the above sequences were shown to be capable to identify the bacteriophage gene pool present both in the phage and bacterial genomes and their mosaic structure. (en)
Title	Multilocus PCR typing strategy for differentiation of Staphylococcus aureus siphoviruses reflecting their modular genome structure Multilocus PCR typing strategy for differentiation of Staphylococcus aureus siphoviruses reflecting their modular genome structure (en)
skos:prefLabel	Multilocus PCR typing strategy for differentiation of Staphylococcus aureus siphoviruses reflecting their modular genome structure Multilocus PCR typing strategy for differentiation of Staphylococcus aureus siphoviruses reflecting their modular genome structure (en)
skos:notation	RIV/00216224:14310/10:00047719!RIV11-GA0-14310___
http://linked.open...avai/riv/aktivita	P R S V Z
http://linked.open...avai/riv/aktivity	P(GA310/09/0459), R, S, V, Z(MSM0021622415)
http://linked.open...iv/cisloPeriodika	9
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Holochová, Pavla Doškař, Jiří Růžičková, Vladislava Pantůček, Roman Rájová, Jana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	273126
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/10:00047719
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	bacteriophage; Staphylococcus aureus; Siphoviridae; molecular typing; PCR; modular genome; evolution (en)
http://linked.open.../riv/klicoveSlovo	Siphoviridae modular genome molecular typing PCR Staphylococcus aureus evolution bacteriophage
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[373D3DD510DE]
http://linked.open...i/riv/nazevZdroje	Environmental Microbiology
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Role of bacteriophages in horizontal transfer of virulence and drug resistance genes in Staphylococcus aureus
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	12
http://linked.open...iv/tvurceVysledku	Doškař, Jiří Pantůček, Roman Růžičková, Vladislava Holochová, Pavla Rájová, Jana Goerke, Christiane
http://linked.open...ain/vavai/riv/wos	000281556900013
http://linked.open...n/vavai/riv/zamer	Molecular basis of cell and tissue regulations
issn	1462-2912
number of pages	12 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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