About: On-line drug metabolites generation and their subsequent target analysis by capillary zone electrophoresis with UV-absorption detection

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About: On-line drug metabolites generation and their subsequent target analysis by capillary zone electrophoresis with UV-absorption detection Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	This study presents the in-capillary enzymatic biotransformation of dextromethorphan, an antitusive drug and opioid receptors antagonist, and subsequent electrophoretic separation of its products. The study includes the optimization of separation parameters to fulfill the requirements of an online microreaction. The analyses were performed in a bare fused-silica capillary using 100mM sodium tetraborate (pH 10.0) mixed with linear polyacrylamide (20%, v/v) and 2-propanol (10%, v/v). This BGE was suitable for monitoring both off-line and in-capillary incubations. The partial filling technique enabled the enzymatic reaction to be carried out in its optimal environment (20mM sodium phosphate, pH 7.4). Finally, in-capillary microreaction in the presence of cytochrome P450 3A4 gave satisfactory outcomes. This study presents the in-capillary enzymatic biotransformation of dextromethorphan, an antitusive drug and opioid receptors antagonist, and subsequent electrophoretic separation of its products. The study includes the optimization of separation parameters to fulfill the requirements of an online microreaction. The analyses were performed in a bare fused-silica capillary using 100mM sodium tetraborate (pH 10.0) mixed with linear polyacrylamide (20%, v/v) and 2-propanol (10%, v/v). This BGE was suitable for monitoring both off-line and in-capillary incubations. The partial filling technique enabled the enzymatic reaction to be carried out in its optimal environment (20mM sodium phosphate, pH 7.4). Finally, in-capillary microreaction in the presence of cytochrome P450 3A4 gave satisfactory outcomes. (en)
Title	On-line drug metabolites generation and their subsequent target analysis by capillary zone electrophoresis with UV-absorption detection On-line drug metabolites generation and their subsequent target analysis by capillary zone electrophoresis with UV-absorption detection (en)
skos:prefLabel	On-line drug metabolites generation and their subsequent target analysis by capillary zone electrophoresis with UV-absorption detection On-line drug metabolites generation and their subsequent target analysis by capillary zone electrophoresis with UV-absorption detection (en)
skos:notation	RIV/00216224:14310/10:00044833!RIV11-GA0-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GAP206/10/0057), P(LC06023), Z(MSM0021622413)
http://linked.open...iv/cisloPeriodika	19
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Glatz, Zdeněk Zeisbergerová, Marta Řemínek, Roman Mádr, Aleš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	276993
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/10:00044833
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	CE; Cytochrome P450; Dextromethorphan; Metabolism (en)
http://linked.open.../riv/klicoveSlovo	Cytochrome P450 Metabolism CE Dextromethorphan
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[5EA43AE25B7E]
http://linked.open...i/riv/nazevZdroje	Electrophoresis
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Integrated bioanalytical technologies for microanalyses and diagnostics with laser induced fluorescence and mass spectrometry coupling. Miniaturised on-line drug-metabolism system based on capillary electrophoresis
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	31
http://linked.open...iv/tvurceVysledku	Glatz, Zdeněk Zeisbergerová, Marta Řemínek, Roman Mádr, Aleš Hoogmartens, Jos Van Schepdael, Ann
http://linked.open...n/vavai/riv/zamer	Proteiny v metabolismu a při interakci organismů s prostředím
issn	0173-0835
number of pages	7 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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