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  • The crystal structure of the receiver domain (RD) of the histidine kinase CYTOKININ-INDEPENDENT1 (CKI1) from Arabidopsis thaliana has been determined at a resolution of 2.0 Angstroem. Crystals of the recombinant RD of CKI1 have been obtained. The crystals diffracted at beamline BW7B of the DORIS-III storage ring to approx. 2.4 Angstroem.The crystals belong to space group C2221 with unit-cell parameters a=54.46, b=99.82, c=79.94 Angstroem, the asymmetric unit contains one molecule of the protein. The structure of CKI1RD had been solved by a molecular-replacement. Initial R value of 0.54, which decreased to R = 0.413 and Rfree = 0.426 after 30 cycles of REFMAC refinement. The three-dimensional structure of A. thaliana CKI1RD shows the conformational conservation of receiver proteins, such as CheY, CheB, ETRRD. CKI1RD is a single domain protein folded in a Rossmann manner with a central beta-sheet formed from five beta-strands and surrounded by sides by two and three alpha-helices.
  • The crystal structure of the receiver domain (RD) of the histidine kinase CYTOKININ-INDEPENDENT1 (CKI1) from Arabidopsis thaliana has been determined at a resolution of 2.0 Angstroem. Crystals of the recombinant RD of CKI1 have been obtained. The crystals diffracted at beamline BW7B of the DORIS-III storage ring to approx. 2.4 Angstroem.The crystals belong to space group C2221 with unit-cell parameters a=54.46, b=99.82, c=79.94 Angstroem, the asymmetric unit contains one molecule of the protein. The structure of CKI1RD had been solved by a molecular-replacement. Initial R value of 0.54, which decreased to R = 0.413 and Rfree = 0.426 after 30 cycles of REFMAC refinement. The three-dimensional structure of A. thaliana CKI1RD shows the conformational conservation of receiver proteins, such as CheY, CheB, ETRRD. CKI1RD is a single domain protein folded in a Rossmann manner with a central beta-sheet formed from five beta-strands and surrounded by sides by two and three alpha-helices. (en)
Title
  • Crystal structure of the receiver domain of the histidine kinase CKI1 from Arabidopsis thaliana
  • Crystal structure of the receiver domain of the histidine kinase CKI1 from Arabidopsis thaliana (en)
skos:prefLabel
  • Crystal structure of the receiver domain of the histidine kinase CKI1 from Arabidopsis thaliana
  • Crystal structure of the receiver domain of the histidine kinase CKI1 from Arabidopsis thaliana (en)
skos:notation
  • RIV/00216224:14310/09:00039824!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita
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  • P(LC06034), Z(MSM0021622415)
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  • 308609
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  • RIV/00216224:14310/09:00039824
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  • histidinkinase CKI1 Arabidopsis cytokinin signaling crystal structure (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [D582309F669F]
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  • Hejátko, Jan
  • Janda, Lubomír
  • Marek, Jaromír
  • Pekárová, Blanka
  • Klumpler, Tomáš
  • Borkovcová, Pavla
http://linked.open...n/vavai/riv/zamer
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  • 14310
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