About: Spectroscopic study of protonation of oligonucleotides containing adenine and cytosine

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Spectroscopic study of protonation of oligonucleotides containing adenine and cytosine Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Acidobasic properties of purine and pyrimidine bases (adenine, cytosine) and relevant nucleosides (adenosine, cytidine) were studied by means of glass-electrode potentiometry and the respective dissociation constants were determined under given experimental conditions (I = 0.1 M (NaCl), t = (25.0 +/- 0.1) degrees of Celsius): adenine (pK_HL = 9.65 +/- 0.04, pK_H2L = 4.18 +/- 0.04), adenosine (pK_H2L = 3.59 +/- 0.05), cytosine (pK_H2L = 4.56 +/- 0.01), cytidine (pK_H2L = 4.16 +/- 0.02). In addition, thermodynamic parameters for bases were calculated. Acidobasic behavior of oligonucleotides ((CAC)3, A3C6, C3A3C3) was studied under the same experimental conditions by molecular absorption spectroscopy. pH-dependent spectral datasets were analyzed by means of advanced chemometric techniques (EFA, MCR-ALS) and the presence of hemiprotonated species concerning (C-C+) a non-canonical pair (i-motif) in titled oligonucleotides was proposed in order to explain experimental data obtained according to literature. Acidobasic properties of purine and pyrimidine bases (adenine, cytosine) and relevant nucleosides (adenosine, cytidine) were studied by means of glass-electrode potentiometry and the respective dissociation constants were determined under given experimental conditions (I = 0.1 M (NaCl), t = (25.0 +/- 0.1) degrees of Celsius): adenine (pK_HL = 9.65 +/- 0.04, pK_H2L = 4.18 +/- 0.04), adenosine (pK_H2L = 3.59 +/- 0.05), cytosine (pK_H2L = 4.56 +/- 0.01), cytidine (pK_H2L = 4.16 +/- 0.02). In addition, thermodynamic parameters for bases were calculated. Acidobasic behavior of oligonucleotides ((CAC)3, A3C6, C3A3C3) was studied under the same experimental conditions by molecular absorption spectroscopy. pH-dependent spectral datasets were analyzed by means of advanced chemometric techniques (EFA, MCR-ALS) and the presence of hemiprotonated species concerning (C-C+) a non-canonical pair (i-motif) in titled oligonucleotides was proposed in order to explain experimental data obtained according to literature. (en)
Title	Spectroscopic study of protonation of oligonucleotides containing adenine and cytosine Spectroscopic study of protonation of oligonucleotides containing adenine and cytosine (en)
skos:prefLabel	Spectroscopic study of protonation of oligonucleotides containing adenine and cytosine Spectroscopic study of protonation of oligonucleotides containing adenine and cytosine (en)
skos:notation	RIV/00216224:14310/09:00037430!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	P S
http://linked.open...avai/riv/aktivity	P(LC06035), S
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Vojtylová, Terézia Trnková, Libuše Lubal, Přemysl Pilařová, Iveta Farková, Marta Táborský, Petr Třísková, Olga Fialová, Dana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	342909
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/09:00037430
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	oligonucleotides; adenine; cytosine; protonation; thermodynamics; chemometrics (en)
http://linked.open.../riv/klicoveSlovo	protonation thermodynamics chemometrics adenine cytosine oligonucleotides
http://linked.open...odStatuVydavatele	SK - Slovenská republika
http://linked.open...ontrolniKodProRIV	[8A8F2F54BDA2]
http://linked.open...i/riv/nazevZdroje	Chem. Papers
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	8 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine.
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	63
http://linked.open...iv/tvurceVysledku	Lubal, Přemysl Trnková, Libuše Pilařová, Iveta Farková, Marta Táborský, Petr Třísková, Olga Dospivová, Dana Vojtylová, Terézia
issn	0366-6352
number of pages	7 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 77 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software