About: Investigation of Thermal Denaturation of Barley Nonspecific Lipid Transfer Protein 1 (ns-LTP1b) by Nuclear Magnetic Resonance and Differential Scanning Calorimetry     Goto   Sponge   NotDistinct   Permalink

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  • Process of thermal denaturation of covalently modified form of barley grain non-specific lipid transfer protein 1b (ns-LTP1b) was investigated by nuclear magnetic resonance (NMR) and differential scanning calorimetry (DSC) up to 115\,$^\circ$C. The denaturation was found to be irreversible and highly cooperative. A method of numerical quantitative analysis allowing to fit the NMR data to a transition-state model without further simplification was developed. Based on the obtained values of transition state enthalpy and entropy, rate of denaturation was calculated as a simple measure of protein stability at various temperatures. Effect of disulfide bond reduction on thermal denaturation of ns-LTP1b was studied and discussed in the context of quality control of barley products during storage and processing.
  • Process of thermal denaturation of covalently modified form of barley grain non-specific lipid transfer protein 1b (ns-LTP1b) was investigated by nuclear magnetic resonance (NMR) and differential scanning calorimetry (DSC) up to 115\,$^\circ$C. The denaturation was found to be irreversible and highly cooperative. A method of numerical quantitative analysis allowing to fit the NMR data to a transition-state model without further simplification was developed. Based on the obtained values of transition state enthalpy and entropy, rate of denaturation was calculated as a simple measure of protein stability at various temperatures. Effect of disulfide bond reduction on thermal denaturation of ns-LTP1b was studied and discussed in the context of quality control of barley products during storage and processing. (en)
Title
  • Investigation of Thermal Denaturation of Barley Nonspecific Lipid Transfer Protein 1 (ns-LTP1b) by Nuclear Magnetic Resonance and Differential Scanning Calorimetry
  • Investigation of Thermal Denaturation of Barley Nonspecific Lipid Transfer Protein 1 (ns-LTP1b) by Nuclear Magnetic Resonance and Differential Scanning Calorimetry (en)
skos:prefLabel
  • Investigation of Thermal Denaturation of Barley Nonspecific Lipid Transfer Protein 1 (ns-LTP1b) by Nuclear Magnetic Resonance and Differential Scanning Calorimetry
  • Investigation of Thermal Denaturation of Barley Nonspecific Lipid Transfer Protein 1 (ns-LTP1b) by Nuclear Magnetic Resonance and Differential Scanning Calorimetry (en)
skos:notation
  • RIV/00216224:14310/09:00036779!RIV11-MSM-14310___
http://linked.open...avai/riv/aktivita
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  • P(1M0570), P(LC06030), Z(AV0Z40310501), Z(MSM0021622413)
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  • 18
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  • 320345
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  • RIV/00216224:14310/09:00036779
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  • Non-specific lipid transfer protein 1; barley grain; thermaldenaturation; nuclear magnetic resonance; differential scanningcalorimetry (en)
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  • US - Spojené státy americké
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  • [151749BA99D7]
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  • Journal of Agricultural and Food Chemistry
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  • 57
http://linked.open...iv/tvurceVysledku
  • Sklenář, Vladimír
  • Wimmerová, Michaela
  • Žídek, Lukáš
  • Chmelík, Josef
  • Žídková, Jitka
  • Matejková, Michaela
http://linked.open...ain/vavai/riv/wos
  • 000269747500053
http://linked.open...n/vavai/riv/zamer
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  • 0021-8561
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  • 14310
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