About: Capillary zone electrophoresis with field enhanced sample stacking as a tool for targeted metabolome analysis of adenine nucleotides and coenzymes in Paracoccus denitrificans

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About: Capillary zone electrophoresis with field enhanced sample stacking as a tool for targeted metabolome analysis of adenine nucleotides and coenzymes in Paracoccus denitrificans Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The main aim of this work was to demonstrate the applicability of capillary zone electrophoresis in combination with field enhanced sample stacking in targeted metabolome analyses of adenine nucleotides - AMP, ADP, ATP, coenzymes NAD+, NADP+ and their reduced forms in Paracoccus denitrificans. 100 mM sodium carbonate/hydrogencarbonate buffer (pH 9.6) with the addition of beta-cyclodextrin at a concentration of 10 mM was found to be an effective background electrolyte for their separation within 20 minutes. Besides this, special attention was paid to the development of the procedure for the extraction of specific metabolites from the bacterium Paracoccus denitrificans. This procedure was not only optimised to achieve the highest metabolite yields but also to obtain a sample that was fully compatible with the on-line preconcetration strategy used. The developed methodology was finally applied in a study of the bacterium P. denitrificans at various stages of the active respiratory chain. The main aim of this work was to demonstrate the applicability of capillary zone electrophoresis in combination with field enhanced sample stacking in targeted metabolome analyses of adenine nucleotides - AMP, ADP, ATP, coenzymes NAD+, NADP+ and their reduced forms in Paracoccus denitrificans. 100 mM sodium carbonate/hydrogencarbonate buffer (pH 9.6) with the addition of beta-cyclodextrin at a concentration of 10 mM was found to be an effective background electrolyte for their separation within 20 minutes. Besides this, special attention was paid to the development of the procedure for the extraction of specific metabolites from the bacterium Paracoccus denitrificans. This procedure was not only optimised to achieve the highest metabolite yields but also to obtain a sample that was fully compatible with the on-line preconcetration strategy used. The developed methodology was finally applied in a study of the bacterium P. denitrificans at various stages of the active respiratory chain. (en)
Title	Capillary zone electrophoresis with field enhanced sample stacking as a tool for targeted metabolome analysis of adenine nucleotides and coenzymes in Paracoccus denitrificans Capillary zone electrophoresis with field enhanced sample stacking as a tool for targeted metabolome analysis of adenine nucleotides and coenzymes in Paracoccus denitrificans (en)
skos:prefLabel	Capillary zone electrophoresis with field enhanced sample stacking as a tool for targeted metabolome analysis of adenine nucleotides and coenzymes in Paracoccus denitrificans Capillary zone electrophoresis with field enhanced sample stacking as a tool for targeted metabolome analysis of adenine nucleotides and coenzymes in Paracoccus denitrificans (en)
skos:notation	RIV/00216224:14310/09:00035508!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(LC06023), Z(MSM0021622413)
http://linked.open...iv/cisloPeriodika	14
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Kučera, Igor Musilová, Jindra Glatz, Zdeněk Sedláček, Vojtěch
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	305849
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/09:00035508
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Adenine Nucleotides and Coenzymes; Capillary zone electrophoresis; Field enhanced sample stacking; Metabolomics; Paracoccus denitrificans (en)
http://linked.open.../riv/klicoveSlovo	Paracoccus denitrificans Capillary zone electrophoresis Metabolomics Adenine Nucleotides and Coenzymes Field enhanced sample stacking
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[D6EAF9CEA825]
http://linked.open...i/riv/nazevZdroje	Journal of Separation Science
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Integrated bioanalytical technologies for microanalyses and diagnostics with laser induced fluorescence and mass spectrometry coupling.
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	32
http://linked.open...iv/tvurceVysledku	Kučera, Igor Glatz, Zdeněk Musilová, Jindra Sedláček, Vojtěch
http://linked.open...ain/vavai/riv/wos	000268595800011
http://linked.open...n/vavai/riv/zamer	Proteiny v metabolismu a při interakci organismů s prostředím
issn	1615-9306
number of pages	5 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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