About: Identification of genes participating in the regulation of CKI1 expression using forward genetic approach

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Overexpression of a hybrid histidine kinase CKI1 results into cytokinin-independent activation of two-component signaling pathway and cytokinin-like response in Arabidopsis hypocotyl explants. CKI1 was found to be involved in the female gametophyte development and procambium activity during vascular tissue formation. Recent data suggest that regulation of CKI1 expression might contribute to the regulation of twocomponent signaling. We used forward genetics approach to identify the factors regulating the activity of CKI1 promoter. Stable transgenic lines carrying ProCKI1:uidA construct were mutated using EMS and M2 seedlings were screened for changes in the GUS pattern. To eliminate constraints of the rate-limiting factor that is a microscopy of the screened mutant lines, we have adopted automated microscopy using the dotSlide system. The level of CKI1 gene expression in mutant lines was tested using qRT-PCR and the more detailed analysis of selected mutants is in the progress. Overexpression of a hybrid histidine kinase CKI1 results into cytokinin-independent activation of two-component signaling pathway and cytokinin-like response in Arabidopsis hypocotyl explants. CKI1 was found to be involved in the female gametophyte development and procambium activity during vascular tissue formation. Recent data suggest that regulation of CKI1 expression might contribute to the regulation of twocomponent signaling. We used forward genetics approach to identify the factors regulating the activity of CKI1 promoter. Stable transgenic lines carrying ProCKI1:uidA construct were mutated using EMS and M2 seedlings were screened for changes in the GUS pattern. To eliminate constraints of the rate-limiting factor that is a microscopy of the screened mutant lines, we have adopted automated microscopy using the dotSlide system. The level of CKI1 gene expression in mutant lines was tested using qRT-PCR and the more detailed analysis of selected mutants is in the progress. (en)
Title	Identification of genes participating in the regulation of CKI1 expression using forward genetic approach Identification of genes participating in the regulation of CKI1 expression using forward genetic approach (en)
skos:prefLabel	Identification of genes participating in the regulation of CKI1 expression using forward genetic approach Identification of genes participating in the regulation of CKI1 expression using forward genetic approach (en)
skos:notation	RIV/00216224:14310/09:00029985!RIV11-GA0-14310___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(GD204/08/H054), P(LC06034), S, Z(MSM0021622415)
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Hejátko, Jan Dobisová, Tereza
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	318336
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/09:00029985
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	CKI1; forward genetic; EMS mutagenesis (en)
http://linked.open.../riv/klicoveSlovo	CKI1 EMS mutagenesis forward genetic
http://linked.open...ontrolniKodProRIV	[8FDB61971454]
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Molecular mechanisms of the cell proliferation and differentiation Regulation of morphogenesis of plant cells and organs
http://linked.open...UplatneniVysledku	2009
http://linked.open...iv/tvurceVysledku	Hejátko, Jan Dobisová, Tereza
http://linked.open...n/vavai/riv/zamer	Molecular basis of cell and tissue regulations
http://localhost/t...ganizacniJednotka	14310

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