About: Functional significance of the Rad51-Srs2 complex in Rad51 presynaptic filament disruption.

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About: Functional significance of the Rad51-Srs2 complex in Rad51 presynaptic filament disruption. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The SRS2 (Suppressor of RAD Six screen mutant 2) gene encodes an ATP-dependent DNA helicase that regulates homologous recombination in Saccharomyces cerevisiae. Mutations in SRS2 result in a hyper-recombination phenotype, sensitivity to DNA damaging agents and synthetic lethality with mutations that affect DNA metabolism. Several of these phenotypes can be suppressed by inactivating genes of the RAD52 epistasis group that promote homologous recombination, implicating inappropriate recombination as the underlying cause of the mutant phenotype. Consistent with the genetic data, purified Srs2 strongly inhibits Rad51-mediated recombination reactions by disrupting the Rad51-ssDNA presynaptic filament. Srs2 interacts with Rad51 in the yeast two-hybrid assay and also in vitro. To investigate the functional relevance of the Srs2-Rad51 complex, we have generated srs2 truncation mutants that retain full ATPase and helicase activities, but differ in their ability to interact with Rad51. The SRS2 (Suppressor of RAD Six screen mutant 2) gene encodes an ATP-dependent DNA helicase that regulates homologous recombination in Saccharomyces cerevisiae. Mutations in SRS2 result in a hyper-recombination phenotype, sensitivity to DNA damaging agents and synthetic lethality with mutations that affect DNA metabolism. Several of these phenotypes can be suppressed by inactivating genes of the RAD52 epistasis group that promote homologous recombination, implicating inappropriate recombination as the underlying cause of the mutant phenotype. Consistent with the genetic data, purified Srs2 strongly inhibits Rad51-mediated recombination reactions by disrupting the Rad51-ssDNA presynaptic filament. Srs2 interacts with Rad51 in the yeast two-hybrid assay and also in vitro. To investigate the functional relevance of the Srs2-Rad51 complex, we have generated srs2 truncation mutants that retain full ATPase and helicase activities, but differ in their ability to interact with Rad51. (en)
Title	Functional significance of the Rad51-Srs2 complex in Rad51 presynaptic filament disruption. Functional significance of the Rad51-Srs2 complex in Rad51 presynaptic filament disruption. (en)
skos:prefLabel	Functional significance of the Rad51-Srs2 complex in Rad51 presynaptic filament disruption. Functional significance of the Rad51-Srs2 complex in Rad51 presynaptic filament disruption. (en)
skos:notation	RIV/00216224:14310/09:00029683!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA301/09/1917), P(GD203/09/H046), P(LC06030), Z(MSM0021622413)
http://linked.open...iv/cisloPeriodika	37
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Krejčí, Lumír
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	315722
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/09:00029683
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	DNA repair; DNA damage; replication; genomic instability (en)
http://linked.open.../riv/klicoveSlovo	replication DNA damage DNA repair genomic instability
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[009E4CE3C136]
http://linked.open...i/riv/nazevZdroje	Nucleic Acids Research
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Biomolecular centre Biochemistry on the crossroad from in silico to in vitro Resolution of replication-recombination DNA intermediates and its role in genomic instability
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	20
http://linked.open...iv/tvurceVysledku	Krejčí, Lumír Changhyun, Seong Eric C., Greene Hannah L., Klein Margaret, Macris-Kiss Olive, Gleeson Patrick, Sung Sierra, Colavito
http://linked.open...ain/vavai/riv/wos	000271819900011
http://linked.open...n/vavai/riv/zamer	Proteiny v metabolismu a při interakci organismů s prostředím
issn	0305-1048
number of pages	10 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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