About: Extraction of PCR-ready DNA from Staphylococcus aureus bacteriophages using carboxyl functionalized magnetic nonporous microspheres

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About: Extraction of PCR-ready DNA from Staphylococcus aureus bacteriophages using carboxyl functionalized magnetic nonporous microspheres Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Magnetic microspheres P(HEMA-co-EDMA) were used for PCR-ready phage DNA isolation from lysogenic strains of Staphylococcus aureus, including two new clinical isolates. The conditions of phage particle lysis were optimized. The quality of eluted phage DNA was evaluated by PCR. It was demonstrated that PCR-ready phage DNA can be isolated from small volumes of phage lysates (150 microL) by magnetic microspheres. The reported method is very expeditious without using toxic compounds such as phenol or chloroform. It can be used for phage identification and phage gene detection. Magnetic microspheres P(HEMA-co-EDMA) were used for PCR-ready phage DNA isolation from lysogenic strains of Staphylococcus aureus, including two new clinical isolates. The conditions of phage particle lysis were optimized. The quality of eluted phage DNA was evaluated by PCR. It was demonstrated that PCR-ready phage DNA can be isolated from small volumes of phage lysates (150 microL) by magnetic microspheres. The reported method is very expeditious without using toxic compounds such as phenol or chloroform. It can be used for phage identification and phage gene detection. (en)
Title	Extraction of PCR-ready DNA from Staphylococcus aureus bacteriophages using carboxyl functionalized magnetic nonporous microspheres Extraction of PCR-ready DNA from Staphylococcus aureus bacteriophages using carboxyl functionalized magnetic nonporous microspheres (en)
skos:prefLabel	Extraction of PCR-ready DNA from Staphylococcus aureus bacteriophages using carboxyl functionalized magnetic nonporous microspheres Extraction of PCR-ready DNA from Staphylococcus aureus bacteriophages using carboxyl functionalized magnetic nonporous microspheres (en)
skos:notation	RIV/00216224:14310/09:00029222!RIV13-GA0-14310___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(GA203/05/2256), P(GA310/09/0459), S, Z(AV0Z40500505), Z(MSM0021622415)
http://linked.open...iv/cisloPeriodika	7
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Španová, Alena Doškař, Jiří Rittich, Bohuslav Pantůček, Roman Horák, Daniel Rájová, Jana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	314479
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/09:00029222
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Magnetic microspheres; P(HEMA-co-EDMA); Staphylococcus aureus; Bacteriophage DNA; Polymerase chain reaction (PCR) (en)
http://linked.open.../riv/klicoveSlovo	Staphylococcus aureus Polymerase chain reaction (PCR) P(HEMA-co-EDMA) Bacteriophage DNA Magnetic microspheres
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[A035347291A8]
http://linked.open...i/riv/nazevZdroje	Journal of Chromatography B
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Role of bacteriophages in horizontal transfer of virulence and drug resistance genes in Staphylococcus aureus Magnetic stimuli-responsive hydrophilic polymer microspheres for biomedicine/bioengineering
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	877
http://linked.open...iv/tvurceVysledku	Doškař, Jiří Horák, Daniel Pantůček, Roman Rittich, Bohuslav Španová, Alena Rájová, Jana
http://linked.open...ain/vavai/riv/wos	000264225200003
http://linked.open...n/vavai/riv/zamer	Progresivní makromolekulární materiály a supramolekulární systémy: syntéza a studium vlastností, jevů a možností využití pro speciální aplikace a moderní technologie Molecular basis of cell and tissue regulations
issn	1570-0232
number of pages	4 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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