About: Integration of on-line protein digestion by trypsin in capillary electrophoresis by means of electrophoretically mediated microanalysis

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About: Integration of on-line protein digestion by trypsin in capillary electrophoresis by means of electrophoretically mediated microanalysis Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In this work, electrophoretically mediated microanalysis (EMMA) was applied to the in-capillary tryptic digestion of proteins for proteomic purposes. Compared to classical in-solution tryptic digestion or the trypsin reactor commonly used for this purpose, the EMMA based method is rapid, can be automated and requires only small amounts of trypsin and protein preparations. Moreover, the protein digestion and the analysis of the resulting peptides are integrated into one procedure. A combination of the EMMA methodology with a partial filling technique was used in this study, since the pH optimum of the trypsin reaction differs strongly from the best pH for the CE separation of peptides. In this setup, part of the capillary is filled with the best buffer for the tryptic digestion (50 mM Tris-HCl buffer, pH 8.5) whereas the rest of the capillary is filled with the background electrolyte optimal for peptide separation (0.1 M phosphate buffer, pH 2.5). In this work, electrophoretically mediated microanalysis (EMMA) was applied to the in-capillary tryptic digestion of proteins for proteomic purposes. Compared to classical in-solution tryptic digestion or the trypsin reactor commonly used for this purpose, the EMMA based method is rapid, can be automated and requires only small amounts of trypsin and protein preparations. Moreover, the protein digestion and the analysis of the resulting peptides are integrated into one procedure. A combination of the EMMA methodology with a partial filling technique was used in this study, since the pH optimum of the trypsin reaction differs strongly from the best pH for the CE separation of peptides. In this setup, part of the capillary is filled with the best buffer for the tryptic digestion (50 mM Tris-HCl buffer, pH 8.5) whereas the rest of the capillary is filled with the background electrolyte optimal for peptide separation (0.1 M phosphate buffer, pH 2.5). (en)
Title	Integration of on-line protein digestion by trypsin in capillary electrophoresis by means of electrophoretically mediated microanalysis Integration of on-line protein digestion by trypsin in capillary electrophoresis by means of electrophoretically mediated microanalysis (en)
skos:prefLabel	Integration of on-line protein digestion by trypsin in capillary electrophoresis by means of electrophoretically mediated microanalysis Integration of on-line protein digestion by trypsin in capillary electrophoresis by means of electrophoretically mediated microanalysis (en)
skos:notation	RIV/00216224:14310/09:00029144!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/06/0047), P(LC06023), Z(MSM0021622413)
http://linked.open...iv/cisloPeriodika	13
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Glatz, Zdeněk Zeisbergerová, Marta
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	319822
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/09:00029144
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Proteomics; Tryptic digestion; Electrophoretically mediated microanalysis; EMMA; Partial filling technique; Sandwich injection (en)
http://linked.open.../riv/klicoveSlovo	Proteomics EMMA Electrophoretically mediated microanalysis Partial filling technique Sandwich injection Tryptic digestion
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[96937E28FFF7]
http://linked.open...i/riv/nazevZdroje	Electrophoresis
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Integrated bioanalytical technologies for microanalyses and diagnostics with laser induced fluorescence and mass spectrometry coupling. Capillary electrophoresis as a tool for the drug-metabolism studies
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	30
http://linked.open...iv/tvurceVysledku	Glatz, Zdeněk Zeisbergerová, Marta Adámková, Andrea
http://linked.open...ain/vavai/riv/wos	000268626900017
http://linked.open...n/vavai/riv/zamer	Proteiny v metabolismu a při interakci organismů s prostředím
issn	0173-0835
number of pages	12 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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