About: Continuous electrochemical monitoring of nitric oxide production in murine macrophage cell line RAW 264.7

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About: Continuous electrochemical monitoring of nitric oxide production in murine macrophage cell line RAW 264.7 Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In this study, we realized the continual and long-term electrochemical detection of NO production by stimulated macrophages using modified porphyrinic micro-sensor. The NO release from RAW 264.7 cells stimulated by lipopolysaccharide started 5 h after the lipopolysaccharide administration. After reaching its maximum at the sixth hour, the stable level of NO production was observed between the seventh and 12th hour of the experiment. A close correlation between the NO signal detected with microelectrode and nitrite accumulation, which had been determined in supernatants removed from stimulated cells, was observed. The presence of iNOS enzyme, which constitutes a main requirement for NO production by stimulated macrophages, was confirmed by Western blot analysis of iNOS protein expression at key time points of the corresponding electrochemical experiment. In this study, we realized the continual and long-term electrochemical detection of NO production by stimulated macrophages using modified porphyrinic micro-sensor. The NO release from RAW 264.7 cells stimulated by lipopolysaccharide started 5 h after the lipopolysaccharide administration. After reaching its maximum at the sixth hour, the stable level of NO production was observed between the seventh and 12th hour of the experiment. A close correlation between the NO signal detected with microelectrode and nitrite accumulation, which had been determined in supernatants removed from stimulated cells, was observed. The presence of iNOS enzyme, which constitutes a main requirement for NO production by stimulated macrophages, was confirmed by Western blot analysis of iNOS protein expression at key time points of the corresponding electrochemical experiment. (en)
Title	Continuous electrochemical monitoring of nitric oxide production in murine macrophage cell line RAW 264.7 Continuous electrochemical monitoring of nitric oxide production in murine macrophage cell line RAW 264.7 (en)
skos:prefLabel	Continuous electrochemical monitoring of nitric oxide production in murine macrophage cell line RAW 264.7 Continuous electrochemical monitoring of nitric oxide production in murine macrophage cell line RAW 264.7 (en)
skos:notation	RIV/00216224:14310/09:00028535!RIV11-AV0-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1M0512), P(1QS500040507), P(GP524/05/P135), Z(AV0Z50040507), Z(AV0Z50040702)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Moravcová, Jana Lojek, Antonín Pekarová, Michaela Kubala, Lukáš Číž, Milan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	308201
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/09:00028535
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Nitric oxide; Macrophages; RAW 264.7; Nitric oxide sensor; Nitrites; iNOS expression (en)
http://linked.open.../riv/klicoveSlovo	Nitric oxide sensor Nitrites iNOS expression Macrophages Nitric oxide RAW 264.7
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[40D2435B1E5C]
http://linked.open...i/riv/nazevZdroje	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Lipid nutrition compounds - modulation of their effects and possibilities of practical application Research center of powdered nanomaterials The formation of nitric oxide in biological systems and possibilities of its modulation
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	394
http://linked.open...iv/tvurceVysledku	Kubala, Lukáš Lojek, Antonín Moravcová, Jana Pekarová, Michaela Hrbáč, Jan Číž, Milan Gregor, Čeněk
http://linked.open...ain/vavai/riv/wos	000266918600029
http://linked.open...n/vavai/riv/zamer	Biophysics of dynamic structures and functions of biological systems Genome and epigenome: 1D and 3D Structure, Dynamics, Interactions with Proteins and Functions
issn	1618-2642
number of pages	8 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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