About: Multidetection platform for microcolumn separation of proteins and peptides

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	This lecture will describe an instrumentation platform based on universal interface for deposition of CE or microLC eluent on a target of a MALDI mass spectrometer. The deposited fractions of proteins or peptides are analyzed off-line using MALDI mass or tandem mass spectrometry (MS or MS/MS) and native laser-induced fluorescence (LIF) detection or subjected to on-target reactions, such as digestion for protein identification. Another detection mode is inductively coupled plasma optical emission and/or mass spectrometry (ICP OES, resp. MS), nature of which is complementary to soft MALDI MS. For the first time, we proposed MALD for desorption of sample from the target and introduction to ICP. Thus, both information about protein identity and content of elements, such as metals or phosphorus will be available from a single separation record. Initial results of MALD - ICP OES/MS analysis of metal species will be shown to demonstrate the feasibility of the new platform. This lecture will describe an instrumentation platform based on universal interface for deposition of CE or microLC eluent on a target of a MALDI mass spectrometer. The deposited fractions of proteins or peptides are analyzed off-line using MALDI mass or tandem mass spectrometry (MS or MS/MS) and native laser-induced fluorescence (LIF) detection or subjected to on-target reactions, such as digestion for protein identification. Another detection mode is inductively coupled plasma optical emission and/or mass spectrometry (ICP OES, resp. MS), nature of which is complementary to soft MALDI MS. For the first time, we proposed MALD for desorption of sample from the target and introduction to ICP. Thus, both information about protein identity and content of elements, such as metals or phosphorus will be available from a single separation record. Initial results of MALD - ICP OES/MS analysis of metal species will be shown to demonstrate the feasibility of the new platform. (en)
Title	Multidetection platform for microcolumn separation of proteins and peptides (en) Multidetection platform for microcolumn separation of proteins and peptides
skos:prefLabel	Multidetection platform for microcolumn separation of proteins and peptides (en) Multidetection platform for microcolumn separation of proteins and peptides
skos:notation	RIV/00216224:14310/08:00033542!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(LC06035), Z(MSM0021622411), Z(MSM0021622412), Z(MSM0021622415)
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Preisler, Jan
http://linked.open.../riv/druhVysledku	A(V) - Audiovizuální tvorba se vzdáleným přístupem
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	381084
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/08:00033542
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	CE; ICP; MALDI MS; interface; off-line coupling (en)
http://linked.open.../riv/klicoveSlovo	MALDI MS ICP interface CE off-line coupling
http://linked.open...i/riv/kodPristupu	L
http://linked.open...ontrolniKodProRIV	[51E0C27E7650]
http://linked.open...i/riv/mistoVydani	Dublin, Irsko
http://linked.open...n/vavai/riv/nosic	PCB
http://linked.open...telVyzkumneZpravy	Dublin City University
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	1 (xsd:int)
http://linked.open...vavai/riv/projekt	Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine.
http://linked.open...UplatneniVysledku	2008
http://linked.open...iv/tvurceVysledku	Preisler, Jan
http://linked.open...rzeVyzkumneZpravy	School of Chemistry Seminar
http://linked.open...n/vavai/riv/zamer	Study and application of plasma chemical reactions in non-isothermic low temperature plasma and its interaction with solid surface Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL) Molecular basis of cell and tissue regulations
http://localhost/t...ganizacniJednotka	14310

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