About: An electrochemical detection of metallothioneins at the zeptomole level in nanolitre volumes     Goto   Sponge   NotDistinct   Permalink

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  • We report on improvement of the adsorptive transfer stripping technique (AdTS) coupled with the differential pulse voltammetry Brdicka reaction to determine a thiol-protein. The current technique has been unable to generate reproducible results when analyzing very low sample volumes (nanolitres). This obstacle can be overcome technically by modifying the current transfer technique including cooling step of the adsorbed analyte. We tested the technique on determination of a promising tumour disease marker protein called metallothionein (MT). The detection limit (3 S/N) of MT was evaluated as 500 zeptomoles per 500 nL (1 pM) and the quantification limit (10 S/N) as 1,500 zeptomoles per 500 nL (3 pM). Further, the improved AdTS technique was utilized to analyze blood serum samples from patients with breast cancer.
  • We report on improvement of the adsorptive transfer stripping technique (AdTS) coupled with the differential pulse voltammetry Brdicka reaction to determine a thiol-protein. The current technique has been unable to generate reproducible results when analyzing very low sample volumes (nanolitres). This obstacle can be overcome technically by modifying the current transfer technique including cooling step of the adsorbed analyte. We tested the technique on determination of a promising tumour disease marker protein called metallothionein (MT). The detection limit (3 S/N) of MT was evaluated as 500 zeptomoles per 500 nL (1 pM) and the quantification limit (10 S/N) as 1,500 zeptomoles per 500 nL (3 pM). Further, the improved AdTS technique was utilized to analyze blood serum samples from patients with breast cancer. (en)
Title
  • An electrochemical detection of metallothioneins at the zeptomole level in nanolitre volumes
  • An electrochemical detection of metallothioneins at the zeptomole level in nanolitre volumes (en)
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  • An electrochemical detection of metallothioneins at the zeptomole level in nanolitre volumes
  • An electrochemical detection of metallothioneins at the zeptomole level in nanolitre volumes (en)
skos:notation
  • RIV/00216224:14310/08:00028434!RIV10-AV0-14310___
http://linked.open...avai/riv/aktivita
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  • P(IAA401990701)
http://linked.open...iv/cisloPeriodika
  • 4
http://linked.open...vai/riv/dodaniDat
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  • 355649
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  • RIV/00216224:14310/08:00028434
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  • metallothionein; voltammetry; biosensor (en)
http://linked.open.../riv/klicoveSlovo
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  • DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV
  • [C4BA670745D6]
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  • Sensors
http://linked.open...in/vavai/riv/obor
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  • 8
http://linked.open...iv/tvurceVysledku
  • Adam, Vojtěch
  • Kizek, René
  • Trnková, Libuše
  • Fabrik, Ivo
  • Baloun, Jiří
http://linked.open...ain/vavai/riv/wos
  • 000256642400013
issn
  • 1424-8220
number of pages
http://localhost/t...ganizacniJednotka
  • 14310
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