About: A determination of metallothionein in larvae of freshwater midges (Chironomus riparius) using Brdicka reaction

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Among wide spectrum of biomolecules induced by various stress factors low molecular mass protein called metallothionein (MT) is suitable for assessment of the heavy metal environmental pollution. The aim of this work was to determine the metallothionein and total thiols content in larvae of freshwater midges (Chironomus riparius) sampled from laboratory exposure to cadmium(II) ions and from field studies using differential pulse voltammetry Brdicka reaction. Unique electrochemical instrument, stationary electrochemical analyser Autolab coupled with autosampler, was utilized for the analysis of the samples. The detection limit for MT was evaluated as 5 nM. The larvae exposed to two doses of cadmium(II) ions for fifteen days under laboratory controlled conditions were at the end of the exposure killed, homogenized and analysed. MT content in control samples was 1.2 microM, in larvae exposed to 50 ng Cd/g it was 2.0 microM and in larvae exposed to 50 microg Cd/g 2.9 microM. Among wide spectrum of biomolecules induced by various stress factors low molecular mass protein called metallothionein (MT) is suitable for assessment of the heavy metal environmental pollution. The aim of this work was to determine the metallothionein and total thiols content in larvae of freshwater midges (Chironomus riparius) sampled from laboratory exposure to cadmium(II) ions and from field studies using differential pulse voltammetry Brdicka reaction. Unique electrochemical instrument, stationary electrochemical analyser Autolab coupled with autosampler, was utilized for the analysis of the samples. The detection limit for MT was evaluated as 5 nM. The larvae exposed to two doses of cadmium(II) ions for fifteen days under laboratory controlled conditions were at the end of the exposure killed, homogenized and analysed. MT content in control samples was 1.2 microM, in larvae exposed to 50 ng Cd/g it was 2.0 microM and in larvae exposed to 50 microg Cd/g 2.9 microM. (en)
Title	A determination of metallothionein in larvae of freshwater midges (Chironomus riparius) using Brdicka reaction A determination of metallothionein in larvae of freshwater midges (Chironomus riparius) using Brdicka reaction (en)
skos:prefLabel	A determination of metallothionein in larvae of freshwater midges (Chironomus riparius) using Brdicka reaction A determination of metallothionein in larvae of freshwater midges (Chironomus riparius) using Brdicka reaction (en)
skos:notation	RIV/00216224:14310/08:00026700!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM0021622412)
http://linked.open...iv/cisloPeriodika	7
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Kostřicová, Zuzana Trnková, Libuše Adam, Vojtěch Kizek, René Hilscherová, Klára Fabrik, Ivo
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	354156
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/08:00026700
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	electrochemistry; heavy metals; metallothionein (en)
http://linked.open.../riv/klicoveSlovo	electrochemistry heavy metals metallothionein
http://linked.open...odStatuVydavatele	CH - Švýcarská konfederace
http://linked.open...ontrolniKodProRIV	[07BAB65F7B15]
http://linked.open...i/riv/nazevZdroje	Sensors
http://linked.open...in/vavai/riv/obor	CG
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	8
http://linked.open...iv/tvurceVysledku	Adam, Vojtěch Hilscherová, Klára Kizek, René Trnková, Libuše Fabrik, Ivo Kostřicová, Zuzana
http://linked.open...ain/vavai/riv/wos	000258180500003
http://linked.open...n/vavai/riv/zamer	Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)
issn	1424-8220
number of pages	14 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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