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rdf:type
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Description
| - Production of soluble forms of cell surface receptors suitable for biochemical and structural studies is critical in modern membrane biology and biochemistry. We have been investigating the soluble forms of the earliest activation antigen of human leukocytes CD69. This receptor is naturally expressed at the cell surface as type II homodimeric membrane protein. However, the elements necessary to prepare the soluble recombinant CD69 suitable for structural studies have been a matter of controversy. We describe here the physical, biochemical and in vivo characteristics of a highly stable soluble form of CD69 obtained by bacterial expression of an optimized extracellular segment corresponding to amino acids Gly70 to Lys199. This optimized protein construct has been derived from the one used previously for CD69 crystallization by its further optimalization with regard to protein stability, solubility, and easy crystallization under conditions promoting ligand binding.
- Production of soluble forms of cell surface receptors suitable for biochemical and structural studies is critical in modern membrane biology and biochemistry. We have been investigating the soluble forms of the earliest activation antigen of human leukocytes CD69. This receptor is naturally expressed at the cell surface as type II homodimeric membrane protein. However, the elements necessary to prepare the soluble recombinant CD69 suitable for structural studies have been a matter of controversy. We describe here the physical, biochemical and in vivo characteristics of a highly stable soluble form of CD69 obtained by bacterial expression of an optimized extracellular segment corresponding to amino acids Gly70 to Lys199. This optimized protein construct has been derived from the one used previously for CD69 crystallization by its further optimalization with regard to protein stability, solubility, and easy crystallization under conditions promoting ligand binding. (en)
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Title
| - Soluble recombinant CD69 receptors optimized to have an exceptional physical and chemicsl stability display prolonged circulation and remain intact in the blood of mice
- Soluble recombinant CD69 receptors optimized to have an exceptional physical and chemicsl stability display prolonged circulation and remain intact in the blood of mice (en)
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skos:prefLabel
| - Soluble recombinant CD69 receptors optimized to have an exceptional physical and chemicsl stability display prolonged circulation and remain intact in the blood of mice
- Soluble recombinant CD69 receptors optimized to have an exceptional physical and chemicsl stability display prolonged circulation and remain intact in the blood of mice (en)
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skos:notation
| - RIV/00216224:14310/08:00026574!RIV11-MSM-14310___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(1M0505), P(IAA500200509), P(IAA5020403), P(LC06030), P(LC07017), P(LC545), Z(AV0Z40500505), Z(AV0Z50200510), Z(AV0Z50520514), Z(MSM0021620808), Z(MSM0021620835), Z(MSM0021622413)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00216224:14310/08:00026574
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - C-type lectin leukocyte activation plasma clearance refolding stability (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Bezouška, Karel
- Brynda, Jiří
- Dohnálek, Jan
- Hofbauerová, Kateřina
- Hudeček, Jiří
- Kadeřávek, Pavel
- Kavan, Daniel
- Novák, Petr
- Sklenář, Vladimír
- Vannucci, Luca
- Vaněk, Ondřej
- Žídek, Lukáš
- Pompach, Petr
- Chmelík, Josef
- Borovičková, Ivana
- Kubíčková, Monika
- Kumar, Vinay
- Gorčík, Lukáš
- Koleno, Petr
- Kopecký, Vladimír, Jr
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http://linked.open...ain/vavai/riv/wos
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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http://localhost/t...ganizacniJednotka
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