About: Molecular analysis of repetitive elements in giant genomes of Fritillaria ( liliaceae)     Goto   Sponge   NotDistinct   Permalink

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  • The genus Fritillaria L. comprises taxa with the largest genomes so far reported for any plant species, and at the same time, it provides extensive infrageneric genome size variation (30,000-127,000 Mb/C) along with surprising karyotype uniformity. The genus has a bicontinental distribution (Eurasia, North America) and both diverged groups independently show a trend of increasing genome size with divergence. We have constructed fosmid genomic DNA libraries from one species of each group with approximately equal genome sizes (~45,000 Mb/C): Fritillaria imperialis (Eurasia) and F. affinis (North America). Both libraries were screened using total genomic DNA as a probe to identify clones containing highly repetitive elements. Four selected clones were sequenced and about 130 kb of repetitive sequences was characterized for each species.
  • The genus Fritillaria L. comprises taxa with the largest genomes so far reported for any plant species, and at the same time, it provides extensive infrageneric genome size variation (30,000-127,000 Mb/C) along with surprising karyotype uniformity. The genus has a bicontinental distribution (Eurasia, North America) and both diverged groups independently show a trend of increasing genome size with divergence. We have constructed fosmid genomic DNA libraries from one species of each group with approximately equal genome sizes (~45,000 Mb/C): Fritillaria imperialis (Eurasia) and F. affinis (North America). Both libraries were screened using total genomic DNA as a probe to identify clones containing highly repetitive elements. Four selected clones were sequenced and about 130 kb of repetitive sequences was characterized for each species. (en)
Title
  • Molecular analysis of repetitive elements in giant genomes of Fritillaria ( liliaceae)
  • Molecular analysis of repetitive elements in giant genomes of Fritillaria ( liliaceae) (en)
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  • Molecular analysis of repetitive elements in giant genomes of Fritillaria ( liliaceae)
  • Molecular analysis of repetitive elements in giant genomes of Fritillaria ( liliaceae) (en)
skos:notation
  • RIV/00216224:14310/08:00025178!RIV11-GA0-14310___
http://linked.open...avai/riv/aktivita
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  • P(GA521/07/0284), Z(MSM0021622415)
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  • 380315
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  • RIV/00216224:14310/08:00025178
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  • Fritillaria; repetitive elements; genomic analysis; genome size (en)
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  • [C22725CAE190]
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  • Lysák, Martin
  • Macas, Jiří
  • Ambrožová, Kateřina
  • Leitch, Ilia J.
http://linked.open...n/vavai/riv/zamer
http://localhost/t...ganizacniJednotka
  • 14310
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