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Description
  • With using bioinformatics tools, several therapeutically famous glycosyltranferases (GTs) have been identified in Mycobacterium tuberculosis genom and genes rv3782 and rv0225 were amplified and cloned into the different expression vectors in order to provide their expression, purification and characterization. The task was complicated by the nature of glycosyltransferases, which are often unstable, hydrophobic and hard to isolate and purify. Therefore, the performance of various expression systems was explored in order to identify the best experimental routes.
  • With using bioinformatics tools, several therapeutically famous glycosyltranferases (GTs) have been identified in Mycobacterium tuberculosis genom and genes rv3782 and rv0225 were amplified and cloned into the different expression vectors in order to provide their expression, purification and characterization. The task was complicated by the nature of glycosyltransferases, which are often unstable, hydrophobic and hard to isolate and purify. Therefore, the performance of various expression systems was explored in order to identify the best experimental routes. (en)
Title
  • USING DIFFERENT APPROACHES IN PREPARATION OF PUTATIVE MYCOBACTERIAL GLYCOSYLTRANSFERASES RV3782 AND RV0225 FOR CRYSTALLOGRAPHIC STUDIES
  • USING DIFFERENT APPROACHES IN PREPARATION OF PUTATIVE MYCOBACTERIAL GLYCOSYLTRANSFERASES RV3782 AND RV0225 FOR CRYSTALLOGRAPHIC STUDIES (en)
skos:prefLabel
  • USING DIFFERENT APPROACHES IN PREPARATION OF PUTATIVE MYCOBACTERIAL GLYCOSYLTRANSFERASES RV3782 AND RV0225 FOR CRYSTALLOGRAPHIC STUDIES
  • USING DIFFERENT APPROACHES IN PREPARATION OF PUTATIVE MYCOBACTERIAL GLYCOSYLTRANSFERASES RV3782 AND RV0225 FOR CRYSTALLOGRAPHIC STUDIES (en)
skos:notation
  • RIV/00216224:14310/08:00024971!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GP203/08/P303), Z(MSM0021622413)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 401837
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/08:00024971
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • tuberculosisů; mycobacterial glycosyltransferases; heterologic expression (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [705F2A6FB95A]
http://linked.open...v/mistoKonaniAkce
  • Athens, Greece
http://linked.open...i/riv/mistoVydani
  • Oxford (UK)
http://linked.open...i/riv/nazevZdroje
  • FEBS Journal Volume 275 Supplement 1
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Kostlánová, Nikola
  • Wimmerová, Michaela
  • Svobodová, Dana
http://linked.open...vavai/riv/typAkce
http://linked.open...ain/vavai/riv/wos
  • 000256633300520
http://linked.open.../riv/zahajeniAkce
http://linked.open...n/vavai/riv/zamer
issn
  • 1742-464X
number of pages
http://purl.org/ne...btex#hasPublisher
  • Blackwell Publishing Ltd.
http://localhost/t...ganizacniJednotka
  • 14310
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