About: Application of Micellar Electrokinetic Capillary Chromatography for Evaluation of Inhibitory Effects on Cytochrome P450 Reaction

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Application of Micellar Electrokinetic Capillary Chromatography for Evaluation of Inhibitory Effects on Cytochrome P450 Reaction Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The major aim of this work is to demonstrate the applicability of micellar electrokinetic capillary chromatography with SDS based pseudostationary phase for the screening of cytochrome P450 inhibitors. In contrast with the other capillary electrophoresis modes the cytochrome P450 reaction mixture thus could be used for the analysis without any pre-treatment. Cytochrome P450 2C9, one of the most important isoforms in human liver, was chosen as a model example for this study in combination with diclofenac as a probe substrate. The inhibitory effect on the given cytochrome P450 reaction was evaluated for two inhibitors with different inhibition potential strong inhibitor sulfaphenazole and moderate inhibitor ketoconazole. As a result 50 % inhibitory concentrations IC50 and inhibition constants Ki were evaluated; their values for both inhibitors were in a good agreement with the literature data determined by different methods. The major aim of this work is to demonstrate the applicability of micellar electrokinetic capillary chromatography with SDS based pseudostationary phase for the screening of cytochrome P450 inhibitors. In contrast with the other capillary electrophoresis modes the cytochrome P450 reaction mixture thus could be used for the analysis without any pre-treatment. Cytochrome P450 2C9, one of the most important isoforms in human liver, was chosen as a model example for this study in combination with diclofenac as a probe substrate. The inhibitory effect on the given cytochrome P450 reaction was evaluated for two inhibitors with different inhibition potential strong inhibitor sulfaphenazole and moderate inhibitor ketoconazole. As a result 50 % inhibitory concentrations IC50 and inhibition constants Ki were evaluated; their values for both inhibitors were in a good agreement with the literature data determined by different methods. (en)
Title	Application of Micellar Electrokinetic Capillary Chromatography for Evaluation of Inhibitory Effects on Cytochrome P450 Reaction Application of Micellar Electrokinetic Capillary Chromatography for Evaluation of Inhibitory Effects on Cytochrome P450 Reaction (en)
skos:prefLabel	Application of Micellar Electrokinetic Capillary Chromatography for Evaluation of Inhibitory Effects on Cytochrome P450 Reaction Application of Micellar Electrokinetic Capillary Chromatography for Evaluation of Inhibitory Effects on Cytochrome P450 Reaction (en)
skos:notation	RIV/00216224:14310/08:00024585!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/06/0047), P(LC06023), Z(MSM0021622413)
http://linked.open...iv/cisloPeriodika	1-2
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Mičíková, Ivana Glatz, Zdeněk Řemínek, Roman Konečný, Jiří
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	356761
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/08:00024585
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Drug metabolism; cytochrome P450; CYP2C9; diclofenac; sulfaphenazole; ketoconazole; MEKC (en)
http://linked.open.../riv/klicoveSlovo	CYP2C9 Drug metabolism MEKC cytochrome P450 diclofenac ketoconazole sulfaphenazole
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[E1A2067F42B9]
http://linked.open...i/riv/nazevZdroje	Journal of Chromatography A
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Integrated bioanalytical technologies for microanalyses and diagnostics with laser induced fluorescence and mass spectrometry coupling. Capillary electrophoresis as a tool for the drug-metabolism studies
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	1189
http://linked.open...iv/tvurceVysledku	Konečný, Jiří Glatz, Zdeněk Řemínek, Roman Mičíková, Ivana
http://linked.open...ain/vavai/riv/wos	000255698000025
http://linked.open...n/vavai/riv/zamer	Proteiny v metabolismu a při interakci organismů s prostředím
issn	0021-9673
number of pages	4 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 48 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software