About: Zeptomole detection of streptavidin using carbon paste electrode and square-wave voltammetry

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About: Zeptomole detection of streptavidin using carbon paste electrode and square-wave voltammetry Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We compared the suitability of avidin and streptavidin for avidin-biotin technology in view of the sensitivity of the analysis using square-wave voltammetry. We found out during our preliminary experiments that streptavidin gave approximately 100 times higher electrochemical response in comparison with avidin at the same experimental conditions and concentration. Thus, we used two approaches for streptavidin determination - analysis directly in electrochemical cell and analysis by adsorptive transfer technique (AdTS). Ten minutes long accumulation on carbon paste electrode surface was ascertained as optimal in both cases. Limits of detection were 0.3 aM (electrochemical cell) and 30 aM (AdTS). We compared the suitability of avidin and streptavidin for avidin-biotin technology in view of the sensitivity of the analysis using square-wave voltammetry. We found out during our preliminary experiments that streptavidin gave approximately 100 times higher electrochemical response in comparison with avidin at the same experimental conditions and concentration. Thus, we used two approaches for streptavidin determination - analysis directly in electrochemical cell and analysis by adsorptive transfer technique (AdTS). Ten minutes long accumulation on carbon paste electrode surface was ascertained as optimal in both cases. Limits of detection were 0.3 aM (electrochemical cell) and 30 aM (AdTS). (en)
Title	Zeptomole detection of streptavidin using carbon paste electrode and square-wave voltammetry Zeptomole detection of streptavidin using carbon paste electrode and square-wave voltammetry (en)
skos:prefLabel	Zeptomole detection of streptavidin using carbon paste electrode and square-wave voltammetry Zeptomole detection of streptavidin using carbon paste electrode and square-wave voltammetry (en)
skos:notation	RIV/00216224:14310/07:00050796!RIV12-MSM-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1M06030), P(LC06035), Z(MSM0021622412)
http://linked.open...iv/cisloPeriodika	11
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Trnková, Libuše Masařík, Michal Potěšil, David
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	462277
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/07:00050796
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	streptavidin; avidin; modified electrode; electrochemistry; protein; carbon paste electrode (en)
http://linked.open.../riv/klicoveSlovo	modified electrode avidin protein carbon paste electrode streptavidin electrochemistry
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[D9A984159E67]
http://linked.open...i/riv/nazevZdroje	Electroanalysis
http://linked.open...in/vavai/riv/obor	CG
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine. Functional genomics and proteomics for crop improvement
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	19
http://linked.open...iv/tvurceVysledku	Adam, Vojtěch Kizek, René Masařík, Michal Trnková, Libuše Petrlová, Jitka Potěšil, David
http://linked.open...ain/vavai/riv/wos	000247105000007
http://linked.open...n/vavai/riv/zamer	Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)
issn	1040-0397
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/elan.200603837
http://localhost/t...ganizacniJednotka	14310

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