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  • Mutual interactions of cytokinins and auxins were identified as a major morphogenetic factor in plants. Molecular mechanisms are still largely unknown. Hypocotyls derived from ethiolated seedlings were affected by various concentration ratios of exogenous auxins and cytokinins. We analysed a role of both phytohormones in an ability of hypocotyl explants to form calli, SAM- and/or RAM-like structures. We applied two auxins, NAA and 2,4-D, differing in the mechanism of their intercellular transport, and auxin transport inhibitor NPA. WUS::NLS-GUS and CLV3::NLS-GUS marker lines were used to recognize the identity of newly formed organs and the role of both phytohormones in the regulation of the cytokinin-to-auxin ratio-dependent developmental switch. Changes in endogenous hormone maxima and position-specific hormone-dependent mitotic cell activity were studied. Suggested model of the cytokinin and auxin interactions during de novo organogenesis in Arabidopsis thaliana will be presented.
  • Mutual interactions of cytokinins and auxins were identified as a major morphogenetic factor in plants. Molecular mechanisms are still largely unknown. Hypocotyls derived from ethiolated seedlings were affected by various concentration ratios of exogenous auxins and cytokinins. We analysed a role of both phytohormones in an ability of hypocotyl explants to form calli, SAM- and/or RAM-like structures. We applied two auxins, NAA and 2,4-D, differing in the mechanism of their intercellular transport, and auxin transport inhibitor NPA. WUS::NLS-GUS and CLV3::NLS-GUS marker lines were used to recognize the identity of newly formed organs and the role of both phytohormones in the regulation of the cytokinin-to-auxin ratio-dependent developmental switch. Changes in endogenous hormone maxima and position-specific hormone-dependent mitotic cell activity were studied. Suggested model of the cytokinin and auxin interactions during de novo organogenesis in Arabidopsis thaliana will be presented. (en)
Title
  • Hormone-dependent de novo organogenesis in Arabidopsis thaliana.
  • Hormone-dependent de novo organogenesis in Arabidopsis thaliana. (en)
skos:prefLabel
  • Hormone-dependent de novo organogenesis in Arabidopsis thaliana.
  • Hormone-dependent de novo organogenesis in Arabidopsis thaliana. (en)
skos:notation
  • RIV/00216224:14310/07:00041969!RIV11-MSM-14310___
http://linked.open...avai/riv/aktivita
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  • P(LC06034), S, Z(MSM0021622415)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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  • 424758
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  • RIV/00216224:14310/07:00041969
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  • arabidopsis; phytohormone interaction; cytokinin; auxin (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [FB70D51E9685]
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  • Hejátko, Jan
  • Pernisová, Markéta
http://linked.open...n/vavai/riv/zamer
http://localhost/t...ganizacniJednotka
  • 14310
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