About: Thin-layer Chromatography and Matrix-assisted Laser Desorption/Ionization Mass Spectrometric Analysis of Oligosaccharides in Biological Samples

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	TLC and MALDI MS of Oligosaccharides in Biological Samples. Thin-layer chromatography (TLC) and matrix-assisted laser desorption/ ionization mass spectrometry (MALDI MS) have been used for analysis of fructooligosaccharides (FOS), used as feed additives (prebiotics), in biological samples. The contents of different regions of a piglet’s intestine (jejunum, ileum, cecum, and colon) were analyzed. Raftilose, a commercially available dietetic product which contains fructooligosaccharides, was added into the feed of the weaning piglets. Complicated biological samples for TLC analysis were used with minimal pretreatment. Analysis was performed on glass-backed precoated silica gel layers impregnated with sodium acetate and developed with butanol–acetic acid–water, 2 + 2 + 1 (v/v), in a vertical glass twin-trough chamber with mobile phase vapor saturation. Visualization of FOS on chromatograms was achieved with a mixture of diphenylamine, aniline, and phosphoric acid in acetone as primary detection reagent. TLC and MALDI MS of Oligosaccharides in Biological Samples. Thin-layer chromatography (TLC) and matrix-assisted laser desorption/ ionization mass spectrometry (MALDI MS) have been used for analysis of fructooligosaccharides (FOS), used as feed additives (prebiotics), in biological samples. The contents of different regions of a piglet’s intestine (jejunum, ileum, cecum, and colon) were analyzed. Raftilose, a commercially available dietetic product which contains fructooligosaccharides, was added into the feed of the weaning piglets. Complicated biological samples for TLC analysis were used with minimal pretreatment. Analysis was performed on glass-backed precoated silica gel layers impregnated with sodium acetate and developed with butanol–acetic acid–water, 2 + 2 + 1 (v/v), in a vertical glass twin-trough chamber with mobile phase vapor saturation. Visualization of FOS on chromatograms was achieved with a mixture of diphenylamine, aniline, and phosphoric acid in acetone as primary detection reagent. (en)
Title	Thin-layer Chromatography and Matrix-assisted Laser Desorption/Ionization Mass Spectrometric Analysis of Oligosaccharides in Biological Samples Thin-layer Chromatography and Matrix-assisted Laser Desorption/Ionization Mass Spectrometric Analysis of Oligosaccharides in Biological Samples (en)
skos:prefLabel	Thin-layer Chromatography and Matrix-assisted Laser Desorption/Ionization Mass Spectrometric Analysis of Oligosaccharides in Biological Samples Thin-layer Chromatography and Matrix-assisted Laser Desorption/Ionization Mass Spectrometric Analysis of Oligosaccharides in Biological Samples (en)
skos:notation	RIV/00216224:14310/07:00025567!RIV11-MSM-14310___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM0021622415)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Preisler, Jan Onofrejová, Lucia
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	455117
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/07:00025567
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	TLC; MALDI MS; oligosaccharides (en)
http://linked.open.../riv/klicoveSlovo	MALDI MS TLC oligosaccharides
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[EC69ED040A76]
http://linked.open...i/riv/nazevZdroje	JPC-J. Planar Chromatogr.-Mod. TLC
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	20
http://linked.open...iv/tvurceVysledku	Preisler, Jan Onofrejová, Lucia Nemcová, Radomíra Podoloničová, Jana Reiffová, Katarina
http://linked.open...ain/vavai/riv/wos	000245524500005
http://linked.open...n/vavai/riv/zamer	Molecular basis of cell and tissue regulations
issn	0933-4173
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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