About: Crystallization and Preliminary Crystallographic Analysis of a Haloalkane Dehalogenase DbjA from Bradyrhizobium japonicum USDA110.

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About: Crystallization and Preliminary Crystallographic Analysis of a Haloalkane Dehalogenase DbjA from Bradyrhizobium japonicum USDA110. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Haloalkane dehalogenases are key enzymes for the degradation of halogenated aliphatic pollutants. A haloalkane dehalogenase, DbjA, constitutes a novel substrate specificity class with high catalytic activity for beta-methylated haloalkanes. In order to reveal the mechanism of the substrate specificity, DbjA has been crystallized by the hanging drop vapor diffusion method. The best crystals were obtained by the microseeding technique with a reservoir solution of 17-19.5% (w/v) PEG 4000, 0.2 M calcium acetate, and 0.1 M Tris-HCl (pH 7.7-8.0). The space group of the DbjA crystal is P212?2 with unit cell parameters a=212.85 A, b=117.84 A, c=55.80 A. The crystal diffracts to 1.75 A resolution. Haloalkane dehalogenases are key enzymes for the degradation of halogenated aliphatic pollutants. A haloalkane dehalogenase, DbjA, constitutes a novel substrate specificity class with high catalytic activity for beta-methylated haloalkanes. In order to reveal the mechanism of the substrate specificity, DbjA has been crystallized by the hanging drop vapor diffusion method. The best crystals were obtained by the microseeding technique with a reservoir solution of 17-19.5% (w/v) PEG 4000, 0.2 M calcium acetate, and 0.1 M Tris-HCl (pH 7.7-8.0). The space group of the DbjA crystal is P212?2 with unit cell parameters a=212.85 A, b=117.84 A, c=55.80 A. The crystal diffracts to 1.75 A resolution. (en)
Title	Crystallization and Preliminary Crystallographic Analysis of a Haloalkane Dehalogenase DbjA from Bradyrhizobium japonicum USDA110. Crystallization and Preliminary Crystallographic Analysis of a Haloalkane Dehalogenase DbjA from Bradyrhizobium japonicum USDA110. (en)
skos:prefLabel	Crystallization and Preliminary Crystallographic Analysis of a Haloalkane Dehalogenase DbjA from Bradyrhizobium japonicum USDA110. Crystallization and Preliminary Crystallographic Analysis of a Haloalkane Dehalogenase DbjA from Bradyrhizobium japonicum USDA110. (en)
skos:notation	RIV/00216224:14310/07:00022814!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(LC06010)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Damborský, Jiří
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	415365
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/07:00022814
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Haloalkane dehalogenases; degradation of halogenated aliphatic pollutants; DbjA; crystals (en)
http://linked.open.../riv/klicoveSlovo	DbjA crystals Haloalkane dehalogenases degradation of halogenated aliphatic pollutants
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[23874543C67D]
http://linked.open...i/riv/nazevZdroje	Acta Crystallographica Section F
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Center of Biocatalysis and Biotransformation
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	63
http://linked.open...iv/tvurceVysledku	Damborský, Jiří Nagata, Yuji Sato, Yukari Natsume, Rio Senda, T. Tsuda, M.
http://linked.open...ain/vavai/riv/wos	000245505800010
issn	1744-3091
number of pages	3 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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