About: Antibodies against sulfur mustard and direct piezoelectric immunosensing in an organic solvent

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The development of polyclonal and monoclonal antibodies against sulfur mustard (SM; yperite) and initial experiments towards the piezoelectric immunosensor are reported. The antibodies were produced using bovine serum albumin and thyroglobulin as carrier proteins, the hapten density was 9-11 SM molecules per one molecule of albumin. The obtained antibodies were able to bind to the piezoelectric quartz crystal sensors with immobilized SM; however, the interaction with free SM was very weak in a competitive assay format. For this reason, the sensors with immobilized antibodies were prepared and tested for affinity to SM using toluene as solvent. Specific binding of SM to polyclonal antibodies was observed in dry toluene, the monoclonal antibody 2C1.2 was able to bind SM in toluene saturated with water. The preliminary results seem promising for immunosensing of SM in organic solvents. The development of polyclonal and monoclonal antibodies against sulfur mustard (SM; yperite) and initial experiments towards the piezoelectric immunosensor are reported. The antibodies were produced using bovine serum albumin and thyroglobulin as carrier proteins, the hapten density was 9-11 SM molecules per one molecule of albumin. The obtained antibodies were able to bind to the piezoelectric quartz crystal sensors with immobilized SM; however, the interaction with free SM was very weak in a competitive assay format. For this reason, the sensors with immobilized antibodies were prepared and tested for affinity to SM using toluene as solvent. Specific binding of SM to polyclonal antibodies was observed in dry toluene, the monoclonal antibody 2C1.2 was able to bind SM in toluene saturated with water. The preliminary results seem promising for immunosensing of SM in organic solvents. (en)
Title	Antibodies against sulfur mustard and direct piezoelectric immunosensing in an organic solvent Antibodies against sulfur mustard and direct piezoelectric immunosensing in an organic solvent (en)
skos:prefLabel	Antibodies against sulfur mustard and direct piezoelectric immunosensing in an organic solvent Antibodies against sulfur mustard and direct piezoelectric immunosensing in an organic solvent (en)
skos:notation	RIV/00216224:14310/07:00022212!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(LC06030), P(OBVTUO2003001), P(OSVTUO2006003)
http://linked.open...iv/cisloPeriodika	7
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Skládal, Petr Přibyl, Jan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	410121
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/07:00022212
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Sulfur mustard; antibody; immunoassay in organic phase; piezoelectric (en)
http://linked.open.../riv/klicoveSlovo	Sulfur mustard antibody piezoelectric immunoassay in organic phase
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[97B78490BC93]
http://linked.open...i/riv/nazevZdroje	Analytical Letters
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Biomolecular centre Imunosensors of CWA and BWA BIODETEKCE - Research of the new methods related to detection as well as identification of warfare biological agents
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	40
http://linked.open...iv/tvurceVysledku	Skládal, Petr Přibyl, Jan Šafář, Bohuslav
issn	0003-2719
number of pages	13 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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