About: Diagnosis of tularemia using piezoelectric biosensor technology

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A piezoelectric immunosensor for indirect diagnosis of tularemic infection in mouse serum was developed. Francisella tularensis LVS antigen was covalently immobilized on the sensing surface using cystamine and glutaraldehyde for activation and modification of the gold electrode. The normal mouse serum (NMS) and serum prepared from mice immunized by Escherichia coli were used as negative controls providing signal of 28 Hz during a 5 min interaction. The tularemic infectious (immunized) mouse serum (IMS) as sample resulted in the signal above 75 Hz (fifth day after infection). The control sensor containing bovine serum albumin as sensing element provided a signal below 5 Hz with NMS as well IMS. The effects of dilution degree and purification of sera were tested. To improve resolution of the method, sample pretreatment steps such as precipitation with ammonium sulphate and immunoglobulin extraction on CBindTM L and MEP HyperCel columns were tested. R.S.D. of measurements was 2.3% for NMS and 2. A piezoelectric immunosensor for indirect diagnosis of tularemic infection in mouse serum was developed. Francisella tularensis LVS antigen was covalently immobilized on the sensing surface using cystamine and glutaraldehyde for activation and modification of the gold electrode. The normal mouse serum (NMS) and serum prepared from mice immunized by Escherichia coli were used as negative controls providing signal of 28 Hz during a 5 min interaction. The tularemic infectious (immunized) mouse serum (IMS) as sample resulted in the signal above 75 Hz (fifth day after infection). The control sensor containing bovine serum albumin as sensing element provided a signal below 5 Hz with NMS as well IMS. The effects of dilution degree and purification of sera were tested. To improve resolution of the method, sample pretreatment steps such as precipitation with ammonium sulphate and immunoglobulin extraction on CBindTM L and MEP HyperCel columns were tested. R.S.D. of measurements was 2.3% for NMS and 2. (en)
Title	Diagnosis of tularemia using piezoelectric biosensor technology Diagnosis of tularemia using piezoelectric biosensor technology (en)
skos:prefLabel	Diagnosis of tularemia using piezoelectric biosensor technology Diagnosis of tularemia using piezoelectric biosensor technology (en)
skos:notation	RIV/00216224:14310/07:00021740!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(OBVTUO2003001), Z(MSM0021622413)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Skládal, Petr Pohanka, Doc. RNDr. PhD, Miroslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	416951
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/07:00021740
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Piezoelectric biosensor; Francisella tularensis; Bioterrorism; Tularemia; Rapid detection; Immunosensor (en)
http://linked.open.../riv/klicoveSlovo	Immunosensor Tularemia Francisella tularensis Piezoelectric biosensor Bioterrorism Rapid detection
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[BE4702056406]
http://linked.open...i/riv/nazevZdroje	Talanta
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Imunosensors of CWA and BWA
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	71
http://linked.open...iv/tvurceVysledku	Pohanka, Miroslav Skládal, Petr Pavliš, Oto
http://linked.open...n/vavai/riv/zamer	Proteiny v metabolismu a při interakci organismů s prostředím
issn	0039-9140
number of pages	5 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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