About: Isolation of PCR-ready DNA from real samples containing PCR inhibitors

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Hydrophilic magnetic particles coated by carboxyl groups were used for PCR-ready DNA isolation. DNA was adsorbed on magnetic particles in the presence of 8 wt. % of poly(ethylene glycol) (PEG 6,000) and 2 M NaCl concentrations, washed and eluted into TE buffer (10 mM Tris-HCl, pH 7.8; 1 mM EDTA; pH 8.0). The eluted DNA was used directly for PCR amplification and identification of Lactobacillus and Bifidobacterium cells in dairy products (cheese, fermented dairy products) and lyophilisates, Trichophyton cells in mixed cultures and bacterial cells in sediments from Lake Baikal. It was also used in GMO testing in foods with success. Hydrophilic magnetic particles coated by carboxyl groups were used for PCR-ready DNA isolation. DNA was adsorbed on magnetic particles in the presence of 8 wt. % of poly(ethylene glycol) (PEG 6,000) and 2 M NaCl concentrations, washed and eluted into TE buffer (10 mM Tris-HCl, pH 7.8; 1 mM EDTA; pH 8.0). The eluted DNA was used directly for PCR amplification and identification of Lactobacillus and Bifidobacterium cells in dairy products (cheese, fermented dairy products) and lyophilisates, Trichophyton cells in mixed cultures and bacterial cells in sediments from Lake Baikal. It was also used in GMO testing in foods with success. (en)
Title	Isolation of PCR-ready DNA from real samples containing PCR inhibitors Isolation of PCR-ready DNA from real samples containing PCR inhibitors (en)
skos:prefLabel	Isolation of PCR-ready DNA from real samples containing PCR inhibitors Isolation of PCR-ready DNA from real samples containing PCR inhibitors (en)
skos:notation	RIV/00216224:14310/07:00020502!RIV10-MZE-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1G57037), P(GA203/05/2256), Z(AV0Z40500505), Z(MSM0021622415)
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Španová, Alena Rittich, Bohuslav
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	427649
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/07:00020502
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	fungi; Trichophyton; nonporous magnetic hydrophilic microspheres; P(HEMA-co-EDMA); P(HEMA-co-GMA) (en)
http://linked.open.../riv/klicoveSlovo	fungi Trichophyton P(HEMA-co-GMA) P(HEMA-co-EDMA) nonporous magnetic hydrophilic microspheres
http://linked.open...ontrolniKodProRIV	[CAC8EB497A83]
http://linked.open...v/mistoKonaniAkce	Budapest
http://linked.open...i/riv/mistoVydani	Budapest, Hungary
http://linked.open...i/riv/nazevZdroje	Biopowders; Advances in the Powder Technology
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Application of molecular biology methods for the effective use of genetic sources of LAB and the characterisation of functional foods quality. Magnetic stimuli-responsive hydrophilic polymer microspheres for biomedicine/bioengineering
http://linked.open...UplatneniVysledku	2007
http://linked.open...iv/tvurceVysledku	Horák, Daniel Rittich, Bohuslav Španová, Alena Rybnikář, Alois
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2007-09-13 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Progresivní makromolekulární materiály a supramolekulární systémy: syntéza a studium vlastností, jevů a možností využití pro speciální aplikace a moderní technologie Molecular basis of cell and tissue regulations
number of pages	6 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Research Institute of Chemical and Process Enginnering, University of Pannonia, Vesazprém, Hungary
https://schema.org/isbn	978-963-9696-23-5
http://localhost/t...ganizacniJednotka	14310

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