About: A rapid PCR-based test for species identification of two cryptic bats Pipistrellus pipistrellus and P. pygmaeus and its application on museum and dropping samples

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About: A rapid PCR-based test for species identification of two cryptic bats Pipistrellus pipistrellus and P. pygmaeus and its application on museum and dropping samples Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The identification of two cryptic bat species of the genus Pipistrellus using a non-destructive and quick method of multiplex PCR and agarose gel electrophoresis is described. Two primer combinations were able to produce species-specific bands that identified reliably individuals that were previously identified by mtDNA sequencing. Robustness of the method was subsequently successfully tested on 16 randomly selected free-living animals from central Europe (tissue samples obtained from a 3 mm punch of wing-membrane) identified to species on the basis of echolocation calls. Nine out of 15 museum specimens and 100% of fresh faecal samples from seven individuals were also successfully identified by this method. The described method thus provides a good way to routinely distinguish two Pipistrellus species by using non-destructive sampling of living individuals or droppings, and will be used in field studies of their ecology. The identification of two cryptic bat species of the genus Pipistrellus using a non-destructive and quick method of multiplex PCR and agarose gel electrophoresis is described. Two primer combinations were able to produce species-specific bands that identified reliably individuals that were previously identified by mtDNA sequencing. Robustness of the method was subsequently successfully tested on 16 randomly selected free-living animals from central Europe (tissue samples obtained from a 3 mm punch of wing-membrane) identified to species on the basis of echolocation calls. Nine out of 15 museum specimens and 100% of fresh faecal samples from seven individuals were also successfully identified by this method. The described method thus provides a good way to routinely distinguish two Pipistrellus species by using non-destructive sampling of living individuals or droppings, and will be used in field studies of their ecology. (en)
Title	A rapid PCR-based test for species identification of two cryptic bats Pipistrellus pipistrellus and P. pygmaeus and its application on museum and dropping samples A rapid PCR-based test for species identification of two cryptic bats Pipistrellus pipistrellus and P. pygmaeus and its application on museum and dropping samples (en)
skos:prefLabel	A rapid PCR-based test for species identification of two cryptic bats Pipistrellus pipistrellus and P. pygmaeus and its application on museum and dropping samples A rapid PCR-based test for species identification of two cryptic bats Pipistrellus pipistrellus and P. pygmaeus and its application on museum and dropping samples (en)
skos:notation	RIV/00216224:14310/07:00020398!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA206/06/0954), Z(AV0Z60930519), Z(MSM0021622416)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Bryja, Josef Hájková, Petra Řehák, Zdeněk
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	408133
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/07:00020398
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Chiroptera; cytochrome b; mtDNA; sibling species; non-invasive sampling (en)
http://linked.open.../riv/klicoveSlovo	cytochrome b sibling species mtDNA Chiroptera non-invasive sampling
http://linked.open...odStatuVydavatele	PL - Polská republika
http://linked.open...ontrolniKodProRIV	[1EEEC941D75D]
http://linked.open...i/riv/nazevZdroje	Acta Chiropterologica
http://linked.open...in/vavai/riv/obor	EG
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Intraspecific variability of populations of two cryptic bat species of genus Pipistrellus in Central Europe
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	9
http://linked.open...iv/tvurceVysledku	Bryja, Josef Hájková, Petra Řehák, Zdeněk Kaňuch, Peter
http://linked.open...n/vavai/riv/zamer	Biodiverzita a ekologie obratlovců: Strategie ochrany a využívání přírodních populací Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time
issn	1508-1109
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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