About: Integration of short-end injection procedure into EMMA methodology.

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The possibility of integration of the short end injection mode in the EMMA methodology is demonstrated in this work on the kinetic studies of haloalkane dehalogenase and rhodanese enzymatic reactions. The essential validations of the EMMA methods combined with the short end and long end injection modes were performed first to confirm their accuracy. The qualitative and quantitative parameters of both approaches such as repetabilities of migration times and peak areas, limits of detection and correlation coefficients were in acceptable ranges. In addition estimated Michaelis constants for the corresponding substrate(s) were comparable being in accordance with previous literature data. Moreover the ping-pong reaction mechanism of rhodanese reaction was confirmed by means of both injection modes. This combination thus preserves the benefits of these instrumental approaches. Whereas the short-end injection procedure brought 5 6.5 times reduction of the analysis time and 2. The possibility of integration of the short end injection mode in the EMMA methodology is demonstrated in this work on the kinetic studies of haloalkane dehalogenase and rhodanese enzymatic reactions. The essential validations of the EMMA methods combined with the short end and long end injection modes were performed first to confirm their accuracy. The qualitative and quantitative parameters of both approaches such as repetabilities of migration times and peak areas, limits of detection and correlation coefficients were in acceptable ranges. In addition estimated Michaelis constants for the corresponding substrate(s) were comparable being in accordance with previous literature data. Moreover the ping-pong reaction mechanism of rhodanese reaction was confirmed by means of both injection modes. This combination thus preserves the benefits of these instrumental approaches. Whereas the short-end injection procedure brought 5 6.5 times reduction of the analysis time and 2. (en)
Title	Integration of short-end injection procedure into EMMA methodology. Integration of short-end injection procedure into EMMA methodology. (en)
skos:prefLabel	Integration of short-end injection procedure into EMMA methodology. Integration of short-end injection procedure into EMMA methodology. (en)
skos:notation	RIV/00216224:14310/07:00020172!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/06/0047), P(LC06023), Z(MSM0021622413)
http://linked.open...iv/cisloPeriodika	1-2
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Němec, Tomáš Glatz, Zdeněk
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	426955
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/07:00020172
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	CE; EMMA substrate; inhibition; haloalkane dehalogenase (en)
http://linked.open.../riv/klicoveSlovo	inhibition EMMA substrate CE haloalkane dehalogenase
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[0891C6A97929]
http://linked.open...i/riv/nazevZdroje	Journal of Chromatography A
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Integrated bioanalytical technologies for microanalyses and diagnostics with laser induced fluorescence and mass spectrometry coupling. Capillary electrophoresis as a tool for the drug-metabolism studies
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	1155
http://linked.open...iv/tvurceVysledku	Němec, Tomáš Glatz, Zdeněk
http://linked.open...n/vavai/riv/zamer	Proteiny v metabolismu a při interakci organismů s prostředím
issn	0021-9673
number of pages	5 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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