About: Study of recombinant cytochrome P450 2C9 activity with diclofenac by micellar electrokinetic capillary chromatography

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Study of recombinant cytochrome P450 2C9 activity with diclofenac by micellar electrokinetic capillary chromatography Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Cytochrome P450 2C9 (CYP2C9) is one of the most important isoform in human liver involved in the metabolism of a large number of therapeutic agents. The aim of this communication is to demonstrate the applicability of capillary electrophoresis for the determination of the enzymatic activity of CYP2C9 with diclofenac as a probe substrate. Micellar electrokinetic capillary chromatography (MEKC) with SDS as a pseudostationary phase was used for this purpose. Compared to other assays, the MEKC based method is rapid, can be automated, and requires only small quantity of enzymes and substrate. Moreover, the enzymatic reaction can be monitored with high sensitivity and reproducibility even when the reaction mixture is used for the analysis without any pre-treatment. The kinetic study on the given enzymatic reaction was also performed since basic characterization of drug biotranformation generally begins with the enzyme kinetic analysis of metabolite formation. Cytochrome P450 2C9 (CYP2C9) is one of the most important isoform in human liver involved in the metabolism of a large number of therapeutic agents. The aim of this communication is to demonstrate the applicability of capillary electrophoresis for the determination of the enzymatic activity of CYP2C9 with diclofenac as a probe substrate. Micellar electrokinetic capillary chromatography (MEKC) with SDS as a pseudostationary phase was used for this purpose. Compared to other assays, the MEKC based method is rapid, can be automated, and requires only small quantity of enzymes and substrate. Moreover, the enzymatic reaction can be monitored with high sensitivity and reproducibility even when the reaction mixture is used for the analysis without any pre-treatment. The kinetic study on the given enzymatic reaction was also performed since basic characterization of drug biotranformation generally begins with the enzyme kinetic analysis of metabolite formation. (en)
Title	Study of recombinant cytochrome P450 2C9 activity with diclofenac by micellar electrokinetic capillary chromatography Study of recombinant cytochrome P450 2C9 activity with diclofenac by micellar electrokinetic capillary chromatography (en)
skos:prefLabel	Study of recombinant cytochrome P450 2C9 activity with diclofenac by micellar electrokinetic capillary chromatography Study of recombinant cytochrome P450 2C9 activity with diclofenac by micellar electrokinetic capillary chromatography (en)
skos:notation	RIV/00216224:14310/07:00020039!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/06/0047), P(LC06023), Z(MSM0021622413)
http://linked.open...iv/cisloPeriodika	8
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Juřica, Jan Tomandl, Josef Glatz, Zdeněk Konečný, Jiří
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	455959
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/07:00020039
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	drug metabolism; cytochrome P450 2C9; diklofenac; MEKC (en)
http://linked.open.../riv/klicoveSlovo	drug metabolism MEKC cytochrome P450 2C9 diklofenac
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[46DEDE62FBF1]
http://linked.open...i/riv/nazevZdroje	Electrophoresis
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Integrated bioanalytical technologies for microanalyses and diagnostics with laser induced fluorescence and mass spectrometry coupling. Capillary electrophoresis as a tool for the drug-metabolism studies
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	28
http://linked.open...iv/tvurceVysledku	Juřica, Jan Tomandl, Josef Konečný, Jiří Glatz, Zdeněk
http://linked.open...ain/vavai/riv/wos	000246116100009
http://linked.open...n/vavai/riv/zamer	Proteiny v metabolismu a při interakci organismů s prostředím
issn	0173-0835
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 48 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software