About: Resolution of overlapped reduction signals in short hetero-oligonucleotides by elimination voltammetry

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About: Resolution of overlapped reduction signals in short hetero-oligonucleotides by elimination voltammetry Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The resolution of overlapped reduction voltammetric signals of adenine (A) and cytosine (C) residues in short synthetic hetero-oligodeoxynucleotides (ODNs) with different sequences of A and C, but with the same ratio (A/C = 1/3), was carried out by elimination voltammetry with linear scan (EVLS). The EVLS, as a mathematical transformation of voltammetric total currents measured at different scan rates, is capable to yield very sensitive resolution of potentially closed signals, which cannot be separated by common electrochemical methods. The best resolution of A and C signals was observed when the EVLS function eliminating the charging and kinetic currents and conserving the diffusion current was combined with adsorption stripping technique (AdS). The resulting peak-counterpeak signal made it possible not only to separate the responses of A and C residues, but also to follow the dynamics of adsorption processes on mercury electrode surfaces. The resolution of overlapped reduction voltammetric signals of adenine (A) and cytosine (C) residues in short synthetic hetero-oligodeoxynucleotides (ODNs) with different sequences of A and C, but with the same ratio (A/C = 1/3), was carried out by elimination voltammetry with linear scan (EVLS). The EVLS, as a mathematical transformation of voltammetric total currents measured at different scan rates, is capable to yield very sensitive resolution of potentially closed signals, which cannot be separated by common electrochemical methods. The best resolution of A and C signals was observed when the EVLS function eliminating the charging and kinetic currents and conserving the diffusion current was combined with adsorption stripping technique (AdS). The resulting peak-counterpeak signal made it possible not only to separate the responses of A and C residues, but also to follow the dynamics of adsorption processes on mercury electrode surfaces. (en)
Title	Resolution of overlapped reduction signals in short hetero-oligonucleotides by elimination voltammetry Resolution of overlapped reduction signals in short hetero-oligonucleotides by elimination voltammetry (en)
skos:prefLabel	Resolution of overlapped reduction signals in short hetero-oligonucleotides by elimination voltammetry Resolution of overlapped reduction signals in short hetero-oligonucleotides by elimination voltammetry (en)
skos:notation	RIV/00216224:14310/07:00019368!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1H-PK/42), P(GP525/04/P132), P(IAA100040602), P(LC06035), Z(AV0Z50040507), Z(MSM0021622412)
http://linked.open...iv/cisloPeriodika	2-3
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Jelen, František Trnková, Libuše Adam, Vojtěch Kizek, René Mikelová, Radka
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	447561
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/07:00019368
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	reduction signals; short hetero-oligonucleotides; elimination voltammetry (en)
http://linked.open.../riv/klicoveSlovo	short hetero-oligonucleotides elimination voltammetry reduction signals
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[C600AE5FA47A]
http://linked.open...i/riv/nazevZdroje	Electroanalysis
http://linked.open...in/vavai/riv/obor	CG
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Research and development of a new type of electrochemical biosensor for the detection of nucleotide sequences in DNA and of genotoxic agents in the environment. Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine. Study of protection mechanisms for stress-induced by heavy metals New approaches in electrochemical analysis of nucleic acids and oligonucleotides aimed at a ultrasensitive microdetection of DNA and DNA hybridization.
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	19
http://linked.open...iv/tvurceVysledku	Adam, Vojtěch Kizek, René Trnková, Libuše Jelen, František Mikelová, Radka
http://linked.open...n/vavai/riv/zamer	Biophysics of dynamic structures and functions of biological systems Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)
issn	1040-0397
number of pages	8 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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